Role of elavl1a in regulating growth and development in zebrafish
HONG Zipu1, ZHANG Wenzhen1, CHEN Congde2
1.Department of Wound Surgery, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou 325027, China; 2.Department of Pediatric Surgery, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China
HONG Zipu,ZHANG Wenzhen,CHEN Congde. Role of elavl1a in regulating growth and development in zebrafish[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(11): 894-898.
Abstract：Objective: To establish the generation of elavl1a-/- mutant zebrafish with an aim to explore the effect of elavl1a gene on zebrafish survival, growth and development. Methods: We generated elavl1a F0 generation mutant zebrafish by CRISPR-Cas9 technology, then obtained F1 generation zebrafish by crossing with wild-type zebrafish. After genotyping, heterozygous F1 zebrafish was self-bred to produce elavl1a-/- homozygous mutant zebrafish. Finally, gene sequencing, Western blot and the relative quantitative statistics and morphological analysis were performed. Results: Using gene sequencing, we found that the elavl1a-/- mutant zebrafish had five
nucleotides missing in the exon 2, which produced frame-shift mutation, leading to appearance of early stop codon.The elavl1a-/- mutant zebrafish could only encode partial peptide, which lacked elval1a protein expression by WB. Moreover, the survival rate of elavl1a-/- mutant zebrafish was greatly reduced, which did not abide by
Mendelian inheritance, and the zebrafish growth and development was delayed. Conclusion: The elavl1a-/- mutant zebrafish was successfully generated, which provides an insight that the elavl1a gene plays an important role in zebrafish survival and development.
