The characteristics of gene mutations associated with cross-resistance to aminoglycosides and peptides in MDR-TB
XIA Qiang1, LIU Haican2, ZHAO Xiuqin2, WAN Kanglin2, ZHAO Lili2.
1.Hangzhou Di’an Medical Laboratory Center, Hangzhou 310012, China; 2.Institute for Communicable Disease Control and Prevention, State Key Laboratory for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
XIA Qiang,LIU Haican,ZHAO Xiuqin, et al. The characteristics of gene mutations associated with cross-resistance to aminoglycosides and peptides in MDR-TB[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(10): 823-828.
Abstract：Objective: To analyze the association between the cross-resistance of streptomycin (SM),kanamycin (KAN) and capreomycin (CAP) and mutations of genes in muti-drug resistant tuberculosis (MDRTB)isolates. Methods: 125 clinical isolates of MDR-TB were revived, drug susceptibility testing to SM, KANand CAP was performed using proportion method, and PCR and sequencing were conducted for the four selected genes (rrs, tlyA, eis promoter, rpsL). Results: Of 125 MDR-TB strains, 7.2% (9/125) were resistant to SM+ KAN, 3.2% (4/125) resistant to SM+CAP, 2% (2/125) resistant to KAN+CAP, and 4.8% (6/125) were all resistant to SM+KAN+CAP. Sequencing showed that the 33A-G mutation of tlyA gene was found in all 125 MDR-TB strains. Four strains of eis promoter mutation were found, all of which were
found in KAN resistant-relatedstrains. A total of 14 mutations, with 8 types of mutation, were found in the rrs gene mutation, of which the 1401A-G type mutation was found in 5 strains, and the mutation in 530 loop region was found in 3 strains. The sensitivity to SM+KAN resistance detection by rrs gene was 33.33%, the sensitivity to SM+CAP resistance detection was 40%, the sensitivity to KAN+CAP resistance detection was 50%, and the sensitivity to SM+ KAN+CAP resistance detection was 50%. Conclusion: Cross-resistance was found between SM, KAN and CAP.The 530 loop region mutations and 1401A-G mutation in rrs of MTB were associated with the cross-resistance toSM, KAN and CAP. The detection of SM, KAN and CAP cross-resistance by rrs mutation is of significance for reference.
收稿日期: 2022-03-08
基金资助:
tuberculosis|multi drug resistance|cross-resistance|molecular characteristic
