WANG Ruoyan,HU Mingqian,QUQUWenxin, et al. The biological function of fucosyltransferase 2 in prostate cancer cell[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(10): 808-812.
Abstract：Objective: To investigate the effect of fucosyltransferase II (FUT2) on the biological function of prostate cancer cells and its potential mechanism. Methods: The
expression of FUT2 in prostate cancer tissueswas detected by immunohistochemistry. FUT2 knockdown prostate cancer cell line (PC-3) and empty control cell line were constructed by liposome transfection technology. The proliferation of PC-3 cells was detected by MTT and plate cloning formation assay. The migration and invasion of PC-3 cells was detected by transwell assay.Western blot was used to detect the expression levels of FUT2, β-catenin, Cyclin D1 and ICAM-1. Results:
Immunohistochemical analysis showed that FUT2 expression was significantly increased in prostate cancer tissues (P<0.05). Knockdown FUT2 inhibited the proliferation, migration and invasion of prostate cancer cells; meanwhile, Cyclin D1 expression was decreased and ICAM-1 expression was increased (P<0.05). Conclusion:FUT2 can promote the proliferation, migration and invasion of prostate cancer cells.
