Relationship between SECTM1 expression and clinicopathological features of colorectal cancer and itseffect on cell function
LIN Ying1,2, NI Leyi2, YU Hejie2, HU Tingting3, CAI Zhenzhai2, LIN Limiao2.
1.TaizhouFirst People’s Hospital, Taizhou 318020, China; 2.Department of Gastroenterology, the Second AffiliatedHospital of Wenzhou Medical University, Wenzhou 325088, China; 3.Department of Gastroenterology, the FirstAffiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China
LIN Ying,NI Leyi,YU Hejie, et al. Relationship between SECTM1 expression and clinicopathological features of colorectal cancer and itseffect on cell function[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(10): 775-783.
Abstract：Objective: To investigate the expression between the expression of secreted and transmembrane1 (SECTM1) in colorectal cancer (CRC) and clinicopathological features as well as its influence on cell function.Methods: The expression of SECTM1 in CRC and matched adjacent tissue samples of 370 CRC patients from from the Second Affiliated Hospital of Wenzhou Medical University between 2011 and 2017 was evaluated by immunohistochemical method. The HCT116 cell line and DLD-1 cell line transfected with a SECTM1-overexpression lentivirus or SECTM1-siRNA, respectively, were further used to examine the effect of SECTM1 on cell proliferation, apoptosis, cell cycling, and invasion. Results: Immunohistochemical staining showed thatSECTM1 was over-expressed in CRC tissue samples compared with paired adjacent tissue samples. SECTM1 was associated with well-or-poorly differentiated adenocarcinoma classification (P<0.001) and lymph node metastasis (P=0.001), though SECTM1 expression was independent of age (P=0.259), sex (P=0.768), tumour location (P=0.299), tumour size (P=0.458), UICC staging (P=0.105) and CEA level (P=0.202). Furthermore, in vitro experiments showed that in HCT116 cell line, the up-regulation of SECTM1 expression promoted cell proliferation, reduced apoptosis, accelerated the process of cell cycle and increased cell invasion (P<0.05). In theDLD-1 cell line, its down-regulation led to the opposite result (P<0.05). Conclusion: SECTM1, a new potential carcinogen, is highly expressed in CRC tissue, which can promote cell proliferation, reduce apoptosis, accelerate cell cycle process, and increase cell invasion.
