HU Dali,CAO Weihong,ZHU Lili, et al. Experimental study on the construction of animal model of low-temperature minimally invasive cryolipolysis[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(9): 705-711.
Abstract：Objective: To establish a minimally invasive Cryolipolysis model of subcutaneous fat in rats.Methods: The rats were randomly divided into low temperature group (0 ℃ group, -20 ℃ group, -40 ℃ group, -60 ℃ group, -80 ℃ group) and room temperature Control group (Control group). Local cold injury of subcutaneous fat in rats was caused by different low temperature intervention, and fat thickness and pathological changes were observed. Results: B-ultrasonography showed that fat thickness decreased to varying degrees in all low-temperature test groups, with the most obvious reduction in fat thickness. No epidermal damage was found when the temperature was -60 ℃ and the contact time was 60 s, which was in line with the requirements of the Cryolipolysis research model (P<0.01). Pathology showed that with the decrease of temperature after local freezing, the inflammatory reaction in adipose tissue was gradually enhanced, and the infiltration of inflammatory cells and destruction of adipose cells became more obvious (P<0.01). The infiltration of inflammatory cells in adipose tissue reached the peak when the temperature was -60 ℃ and the contact time was 60 s (P<0.01). Immunohistochemistry showed that NF-κB-p65 expression was obvious at week 2 and TGF-β1 expression was obvious at week 4, which were significantly different from control group (P<0.01). When the temperature was as below -20 ℃, the apoptosis of Tunel stained adipocytes increased gradually, and the apoptosis was most obvious when the low temperature (-60 ℃) was intervened 60 s. Conclusion: Under the condition of 60 seconds’ intervention at -60 ℃, a stable minimally invasive Cryolipolysis model of subcutaneous fat in SD rats can be established by using low-temperature probe technology.
