白细胞介素17对大鼠肝星状细胞胶原代谢的影响

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摘要目的：探讨白细胞介素17（IL-17）对大鼠肝星状细胞（HSCs）增殖及基质金属蛋白酶-9（MMP-9）和基质金属蛋白酶组织抑制剂-1（TIMP-1）表达的影响及其与肝纤维化的关系。方法：采用Optiprep密度梯度离心法分离HSCs并进行鉴定、传代。采用免疫细胞化学法检测IL-17作用HSCs 72 h后α肌动蛋白（α-SMA）表达的影响。采用MTT法检测IL-17作用HSCs 24、48和72 h后细胞的增殖情况。采用RT-PCR法和ELISA法分别检测IL-17作用HSCs后MMP-9、TIMP-1 mRNA和蛋白表达的影响。结果：成功分离并培养HSCs，一定浓度的IL-17能促进HSCs α-SMA的表达并且促进HSCs增殖。IL-17能提高HSCs MMP-9和TIMP-1 mRNA和蛋白的分泌，呈浓度依赖性，且TIMP-1/MMP-9比值随IL-17浓度增加而递增。结论：IL-17不仅促进HSCs增殖，还能促进α-SMA、MMP-9和TIMP-1的表达，同时提高TIMP-1与MMP-9的比值，可能在肝纤维化中扮演重要角色。

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	余盼攀1
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关键词 ：白细胞介素17；肝星状细胞；&alpha, 肌白蛋白；基质金属蛋白酶-9；基质金属蛋白酶组织抑制剂-1；大鼠

Abstract：Objective: To investigate the effect of Interleukin17 (IL-17) on proliferation and expression of MMP-9, TIMP-1 in rat hepatic stellate cells in vitro. Methods: The HSCs were isolated by method of Optiprep. Immunocytochemical method was used to detect the α-SMA expression of HSCs. The HSCs was treated with different concentrations of IL-17 and the proliferation of HSCs was measured by MTT. The MMP-9 and TIMP-1 mRNA levels were detected by reverse-transcription polymerase chain reaction (RT-PCR). While the MMP-9 and TIMP-1 protein were measured by ELISA. Results: HSCs were isolated and cultured successfully. Stimulated with IL-17 could significantly increase proliferation and α-SMA expression in HSCs. IL-17 could promote the MMP-9 and TIMP-1 mRNA expression and increased protein secresion in HSCs by dose depedent manner. With increased of IL-17 concentration, the value of TIMP-1/MMP-9 was markedly upregulated. Conclusion: IL-17 can promote the proliferation of HSCs and also increases α-SMA, MMP-9 and TIMP-1 expression, then upregulate the value of TIMP-1/MMP-9 in HSCs, which may play an important role in live fibrosis.

Key words： interleukin17 hepatic stellate cells α-SMA MMP-9 TIMP-1 rats

收稿日期: 2014-05-15

基金资助:浙江省外科学重中之重学科基金资助项目（2008-255）。

作者简介: 余盼攀（1986-），男，浙江衢州人，住院医师，医学硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2014/V44/I12/868

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