ANG Zhiqiang,HUANG Yanyan,HE Hui, et al. Expression of SMARCA5 and its role in non-small cell lung cancer[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(7): 550-556.
Abstract：Objective: To study the relationship between SMARCA5 expression and prognosis of patients with non-small cell lung cancer (NSCLC) and also to investigate the effect of silencing SMARCA5 expression on the biological behavior of NSCLC. Methods: The immunohistochemistry method was adopted to detect the expression of SMARCA5 proteins in NSCLC and normal lung tissues. The correlation between the expression of SMARCA5 and clinicopathological factors and survival prognosis of patients with NSCLC was statistically analyzed. Western blot and reverse transcription polymerase chain reaction were used to detect the expression of SMARCA5 in normal human bronchial epithelial cell line and human lung cancer cell lines (A549, Lu165, SK-MES-1, and NCI-H520). SMARCA5 siRNA was employed to silence the expression of SMARCA5. MTT and Transwell methods were used to detect the proliferation and invasion of lung cancer cells. Results: SMARCA5 protein was highly expressed in 78(59.5%) NSCLC tissues. SMARCA5 expression was significantly associated with poor differentiation (χ2=16.666, P<0.001), TNM stage (χ2=24.462, P<0.001), and pleural invasion (χ2=4.601, P=0.032). Additionally, high expression of SMARCA5 was associated with poor prognosis of patients with NSCLC (Log-rank test, P=0.001). The knockdown of SMARCA5 expression inhibited cell growth (P<0.05) and invasion (P<0.05) in NSCLC. Conclusion: The high expression of SMARCA5 is associated with tumor TNM stage, pleural invasion, poor differentiation and poor prognosis in NSCLC patients, and the knockdown of SMARCA5 expression can reduce the proliferation and invasion activity of NSCLC cells. SMARCA5 may play a decisive role in tumorigenesis of NSCLC.
