XIANG Youqun,FANG Guan,JIN Yixiang, et al. Regulator of G protein signaling 13 plays an inhibitory role in the progression of colorectal cancer via regulating β-catenin pathway[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(6): 440-445.
Abstract：Objective: To explore the role of regulator of G protein signaling 13 (RGS13) in the progression of colorectal cancer (CRC). Methods: The expression of RGS13 in CRC tissues and cells was analyzed at the mRNA level using the TCGA database and real-time quantitative polymerase chain reaction (qPCR). Further analysis at the protein level was performed using immunohistochemistry (IHC) and western blot. The effect of RGS13 on the proliferation, migration and invasion of CRC cells was detected by ATP cell viability assay,soft agar colony formation assay and cell migration and invasion assay. The downstream molecular mechanism was explored by western blot, RT-qPCR and other experiments. Results: The expression of RGS13 was low in CRC tissues and cell lines (P<0.01). The lower the expression of RGS13, the shorter the disease-free survival of patients (P=0.017). Down-regulation of RGS13 could significantly promote the proliferation, migration and invasion of CRC cells (P<0.01), indicating that RGS13 played a tumor suppressor role in the progression of CRC.Mechanistically, RGS13 inhibited CRC by down-regulating the protein level of β-catenin in the Wnt/β-catenin signaling pathway, thereby reducing the expression levels of oncogenes c-Myc, MMP7 and CCND1 (P<0.01).Conclusion: RGS13 may play an important role in inhibiting CRC progression by down-regulating β-catenin.
