1.Department of Pediatric Infectious Disease, the Second Affiliated Hospital &Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China; 2.School of Basic Medicine,Anhui Medical University, Hefei 230000, China
RUAN Yufei,LIU Qi,WU Bo, et al. Establishment and identification of acute hepatitis animal model infected by murine cytomegalovirus in C57BL/6 mice[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(5): 345-351,357.
Abstract：Objective: To establishe the acute hepatitis model of C57BL/6 mouse infected with murine cytomegalovirus (MCMV) and analyze its infectious characteristics so as to identify the model. Methods: Twentyfour C57BL/6 mice were randomized to the negative control group and virus infection group (n=12 in each). Murine cytomegalovirus (MCMV) suspension 1.0×106 PFU (200 μL) was intraperitoneally injected in the virus infection group mice, and an equivalent volume of mouse embryonic fibroblast (MEF) suspension in the negative control group. The levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were tested on day 3 and day 7 after infection. Meanwhile, virus isolation, histopathological analysis, detection of MCMV IE and M55 genes, interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) were performed.Results: In virus infection group, all the liver homogenates were positive for virus isolation and the incidence of hepatitis was 100%. The pathological changes of hepatitis occurred on the 3rd day after infection. Compared with the negative control group, the levels of serum ALT and AST in virus infected group were significantly increased (P<0.01). Focal necroinflammatory lesions were observed in the liver of the virus infected group on day 3 of
hematoxylin-eosin staining, which lasted until day 7. Ishak score was significantly higher than that of the negative control group (P<0.01). MCMV IE and M55 genes were detectable in liver tissue on day 3 after infection, and the IE gene was still detected on day 7 after infection. The mRNA expression of inflammatory cytokines, IL-6, TNF-α and IL-1β were significantly increased on day 3 and day 7 after infection (P<0.05). Conclusion: The acute hepatitis model infected with MCMV in C57BL/6 mice was established successfully, and the infection is mainlymanifested in the early stage of acute infection.
