The role of extracellular HSP70 in regulating lung injury in acute pancreatitis
LI Ying1, DAI Shengjie2,SUN Xuecheng2, KONG Hongru2, CHEN Zimiao3, SUN Hongwei2.
1.Department of Hepatopancreatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China; 2.Department of Operating Room, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China;3.Department of Endocrinology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015,China
LI Ying,DAI Shengjie,SUN Xuecheng, et al. The role of extracellular HSP70 in regulating lung injury in acute pancreatitis[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(3): 216-221.
Abstract：Objective: To investigate the regulating role of extracellular heat shock protein 70 (HSP70) in acute pancreatitis complicated with acute lung injury. Methods: The mouse models of acute pancreatitis with different expression of extracellular HSP70 were constructed. The role of extracellular HSP70 in acute pancreatitis and acute lung injury was observed by detecting pancreatic water content, HE staining of pancreas and lung tissue,enzyme linked immunosorbent assay (ELISA), transcriptome sequencing of lung tissue and RT-PCR. Results:In the experiments of pancreatic water content and HE staining of pancreatic and lung tissues, higher level of extracellular HSP70 showed higher pancreatic water content, and more obvious edema of pancreatic and lung tissues (P<0.05). In the ELISA experiment, higher level of extracellular HSP70 was also accompanied by higher level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) (P<0.05). In the lung tissue transcriptional
sequencing experiment, the gene expression change caused by acute pancreatitis was the most significant, which mainly affected the signal pathways such as ECM-receptor interaction, Cytokine-cytokine receptor interaction,Focal adhesion and PI3K/Akt and thereby changed the function of cell adhesion. In the RT-PCR experiment, the expression of the most significantly changed related genes was consistent with the results of transcriptome sequencing. Conclusion: This study shows that extracellular HSP70 can aggravate lung injury in acute pancreatitis and may play a role through such signal pathways as ECM-receptor interaction, Cytokine-cytokine receptor interaction, Focal adhesion and PI3K/Akt.
