Effects of long-term high salt diet on blood glucose and fecal metabolites in mice
XIE Wei1, ZHENG Jujia1,LI Qinyao1, GAN Jing2, LIN Zhuofeng1.
1.School of Pharmaceutical Science, Wenzhou Medical University,Wenzhou 325035, China; 2. Department of Nephrology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China
XIE Wei,ZHENG Jujia,LI Qinyao, et al. Effects of long-term high salt diet on blood glucose and fecal metabolites in mice[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(3): 173-179.
Abstract：Objective: To explore the mechanism of long-term high salt diet on blood glucose in male C57BL/6J mice by fecal metabonomics. Methods: Eight 8-week-old mice were divided into two groups, four in each. The control group was given ordinary diet and the high salt group was given 8% high salt diet for 22 weeks.After intervention, fasting plasma glucose (FPG), glucose tolerance test (GTT) and insulin tolerance test (ITT) were performed, and tail cuff method was used to monitor the systolic blood pressure and diastolic blood pressure of tail artery in each group. The fecal samples of mice were collected and analyzed by liquid chromatographymass spectrometry (LC-MS). The data pattern was identified by principal component analysis (PCA). Results:Compared with control group, the high salt group significantly decreased body weight (P<0.01), so did the proportion of brown fat (P<0.01). The level of FPG was significantly higher than that in the control group (P<0.05), so was the area under the curve (AUC) of GTT and the systolic blood pressure (P<0.05), but there was no significant difference in the AUC of diastolic blood pressure and ITT (P>0.05). Metabonomic analysis showed differences in the principal components of fecal metabolites between the two groups, which was distinguishable. A total of 12 metabolites including 3-hydroxyoctanoyl carnitine, 2-otenedioic acid, 2-octenoate, drithromycin,NBD-stearoyl-2-arachidonoyl-sn-glycerol, 20-ethyl-PGE2, stevioside, biperiden, lysyl-Methionine, norcholic acid, lansiol, 7, 8, 7’, 8’-tetradehydroastaxanthin and PGP-Me were found and identified. These metabolites were mainly involved in amino acid metabolism, prostaglandin metabolism in islets and oxidative stress inflammation.Conclusion: Long-term high salt diet not only causes abnormal blood glucose metabolism in mice, but also has imbalanced regulation of amino acid metabolism, prostaglandin metabolism in islets and oxidative stress inflammation.
