WU Wenyi,BAI Yongyu,Zhuang Xiaopeng, et al. Expression of hsa_circ_0001322 in gastric cancer and it’s clinical significance[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(2): 104-108.
摘要目的：探讨hsa_circ_0001322在胃癌组织中的表达情况及其临床意义。方法：收集2019年10月至2020年4月温州医科大学附属第一医院收治的54例胃癌患者肿瘤组织及其癌旁正常胃黏膜组织，采用实时定量聚合酶链反应（RT-qPCR）检测胃癌组织及其正常胃黏膜组织中hsa_circ_0001322的相对表达量并分析其与临床病理特征的关系。结果：胃癌组织hsa_circ_0001322相对表达量低于癌旁正常胃黏膜组织（0.309 vs. 1.000，P＜0.05）。临床病理特征关联分析发现淋巴结转移组患者胃癌组织中hsa_circ_0001322的相对表达量低于无淋巴结转移组（0.208 vs. 0.391，P＜0.05），偏相关分析结果表明排除患者年龄、性别、肿瘤大小、肿瘤部位、肿瘤浸润深度和细胞分化程度的影响后，hsa_circ_0001322的表达量与胃癌的淋巴结转移存在负相关（r=-0.587，P＜0.05）。ROC曲线分析结果显示hsa_circ_0001322的相对表达量判断胃癌发生淋巴结转移的曲线下面积（AUC）为0.808，以相对表达量0.254作为截断值时，其敏感度和特异度分别为90.0%和75.0%。结论：hsa_circ_0001322在胃癌组织中相对低表达，并且其表达变化与淋巴结转移显著相关，可能是胃癌诊断和预测淋巴结转移的潜在生物标志物。
Abstract：Objective: To analyze the expression change and diagnostic value of a circular RNA, hsa_circ_0001322 in gastric cancer. Methods: Gastric cancer mucosa tissues and paired normal gastric mucosa tissues of 54 patients were collected in the First Affiliated Hospital of Wenzhou Medical University from October 2019 to April 2020. The expression level of hsa_circ_0001322 was examined using real-time quantitative polymerase chain reaction (RT-qPCR) and its correlation with different clinicopathological factor groups were analyzed. Results: The expression level of hsa_circ_0001322 in gastric cancer tissues was lower than that in normal gastric mucosa tissues (0.309 vs. 1.000, P<0.05). The lower expression of hsa_circ_0001322 was also significantly associated with lymph node (LN) metastasis (0.208 vs. 0.391, P<0.05). Partial correlation analysis showed that the expression level of hsa_circ_0001322 was negatively correlated with LN metastasis of gastric cancer (R=-0.587, P<0.05), after excluding the influence of age, gender, tumor size, tumor location, depth of invasion or cell differentiation. The area under the ROC curve of hsa_circ_0001322 was 0.808, and the sensitivity and specificity of prediction with threshold value at 0.254 was 90.0% and 75%, respectively. Conclusion: Down-regulation of hsa_circ_0001322 was correlated with gastric cancer tissue and lower expression of hsa_circ_0001322 was evidently associated with lymph node metastasis, which suggested that hsa_circ_0001322 may serve as a potential biomarker for diagnosis of gastric cancer and prediction of lymph node metastasis.
