Extraction and identification of endometriosis-associated macrophages
CHEN Sailing, WANG Fang, CHEN Huiqian, HU Xueli, WU Meiling, DUAN Ping
Department of Gynaecology and Obstetrics, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China
CHEN Sailing,WANG Fang,CHEN Huiqian, et al. Extraction and identification of endometriosis-associated macrophages[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(2): 87-91.
Abstract：Objective: To explore the method of extracting endometriosis (EMs)-associated macrophages (EAMs). Methods: Immunohistochemistry staining was used to explore the expression of CD68 and CD163 in pathological sections of ectopic endometrium (Ec) and normal endometrium (En). Collect three cases of EMs patients diagnosed by laparoscopy, separate EAMs by mechanical method combined with type IV collagenase digestion, and use flow cytometry to detect the expression of CD11b, CD163, and CD80. Results: High expression of type 2 macrophages can be seen in EMs tissues. The extracted EMs primary macrophages have the morphological characteristics of macrophages. The double-positive ratio of CD11b and CD163 is 6.46%, and no CD80-positive cell expression is seen. Conclusion: The method used in this study can effectively extract EAMs and improve their purity, which is of great value for further research on the characteristics and biological behavior of endometriosis-associated macrophages.
