Antioxidation of hydrogen-rich water on renal injury induced by type 1 dibetes in mice
1.The First Clinical Medical Academy, Wenzhou Medical University, Wenzhou, 325035; 2.School of Environment Science and Public Health, Wenzhou Medical University, Wenzhou, 325035; 3.Department of Pathophysiology, Wenzhou Medical University, Wenzhou, 325035
ZHAO Chenchen1,WANG Qingqing1,QIU Pengcheng1, et al. Antioxidation of hydrogen-rich water on renal injury induced by type 1 dibetes in mice[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(11): 813-816.
Abstract：Objective: To study the protective effect of hydrogen on renal injury induced by type 1 dibetes in mice and explore the possible mechanism. Methods: Sixty healthy male ICR mice were randomly divided into control group (A), diabetic group (B) and diabetes hydrogen water intervention group (C). Diabetes were induced by intraperitoneal injection of streptozotocin 150 mg/kg. Group C were injected saturated hydrogen-rich water 5 mL/kg and group B were injected with equal normal saline every day for 8 weeks in mice models of type 1 dibetes. The blood was collected from the tail vein and blood glucose was measured. Renal injury were identified by hematoxylin and eosin (HE) staining. Expression of Nox4 and HO-1 were identified by immunohistochemical staining. Results: Expression of Nox4 in Group C was significantly lower than that in group B, expression of HO-1 in Group C was significantly higher than that in group B. Blood glucose in Group B and C were higher than that in Group A, but there was no difference between the two groups. Kidney HE staining did not show significant lesions. Conclusion: Hydrogen-rich water can reduce oxidative stress in renal tissue of diabetic mice.
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