YE Tingyu,HUANG Hang,PAN Yue, et al. Linc00346 promotes proliferation of bladder cancer cells via PI3K/Akt signaling pathway through inhibiting PTEN[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(12): 995-999.
Abstract：Objective: To study the molecular mechanism of linc00346 in promoting proliferation of bladder cancer cells. Methods: Real-time fluorescence quantitative PCR (RT-PCR) was used to detect the expression of linc00346 and PTEN in urothelial carcinoma tissues of bladder. The expression level of linc00346 and PTEN in bladder cancer cell lines T24 was detected by RT-PCR after knockdown linc00346. The expression of PTEN protein and Akt, p-Akt protein in PI3K/Akt signaling pathway was measured by Western blot; The cell proliferation ability was determined by CCK-8 method. Results: The expression of linc00346 was up-regulated, while the PTEN expression level was down-regulated in urothelial carcinoma tissues (P<0.05). After knockdown linc00346 in T24 cells, the expression level of linc00346 decreased, the level of PTEN mRNA increased (P<0.05), the expression of PTEN protein increased, and the expression of p-Akt reduced (P<0.05). When PTEN inhibitor SF1670 was given at the same time, the expression of PTEN protein was down-regulated, and that of p-Akt was up-regulated (P<0.05). The proliferation ability of T24 cells was inhibited obviously after silencing linc00346.Furthermore, SF1670 made the cell proliferation ability increased (P<0.05). Conclusion: Linc00346 can increase the proliferation ability of bladder urothelial carcinoma cells by inhibiting PTEN, which activates PI3K/Akt signaling pathway.
