XIA Lu,HUANG Min,MEI Jie, et al. Ferulic acid inhibits the invasion and migration of cervical cancer HeLa cells by regulating mitochondrial apoptosis in vitro[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(12): 974-979.
Abstract：Objective: To study the effect of Ferulic Acid (FA) on cervical cancer (HeLa) cell lines and its possible mechanism. Methods: HeLa cells were cultured in vitro and divided into the control group and FA treatment group (1, 2 and 4 mmol/L). CCK-8 and scratch test detected cell proliferation and migration ability.Transwell test evaluated cell invasion ability. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of apoptosis-related genes Bcl-2, Bax and Cleaved-caspase-3 in HeLa cells after treatment with different concentrations of FA, and immunofluorescence was used to detect the expression and status of mitochondria in HeLa cells. Results: RT-qPCR results showed that the expression of Bcl-2 gene in HeLa cells gradually decreased, while the expression of Bax and Cleaved-caspase-3 genes gradually increased in a dose-dependent manner, with statistical difference (P<0.05), compared with the control group.Immunofluorescence showed that the content of mitochondria in HeLa cells gradually decreased and the function gradually weakened with statistical difference (P<0.05), compared with the control group. The results of Transwell and scratch experiments showed that different concentrations of FA reduced the number of invaded cells and migrated cells of HeLa cells in a dose-dependent manner with significant differences (P<0.05),compared with the control group. Conclusion: FA inhibits the proliferation, invasion and migration of HeLa cells through the Bcl-2 family protein pathway, and induces mitochondrial-mediated apoptosis.
