Kaempferide inhibits the proliferation and migration of lung cancer cell A549 by down-regulating circ-RPL15
ZHENG Chengchao1, MAO Jiabin1, HE Daqiang2
1.Department of Tuberculosis, the Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China; 2.Department of Laboratory, Hangzhou First People’s Hospital Affiliated to Medical College of Zhejiang University, Hangzhou 310006, China
ZHENG Chengchao,MAO Jiabin,HE Daqiang. Kaempferide inhibits the proliferation and migration of lung cancer cell A549 by down-regulating circ-RPL15[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(12): 960-967.
Abstract：Objective: To explore whether kaempferide can affect the biological behavior of lung cancer cell A549 through circ-RPL15. Methods: Kaempferide of 25, 50, and 75 μg/mL was used to treat lung cancer cells A549. The dual luciferase report experiment was used to analyze the targeted regulation of miR-489-3p by circ-RPL15. circ-RPL15 was silenced or overexpressed in cell A549, and treated with 75 μg/mL kaempferol. Cell counting kit-8 (CCK-8) was applied to measure changes in the viability of lung cancer cells A549 in each group;plate cloning was performed to detect cell clone formation; flow cytometry was used to monitor cell apoptosis;Western blot was employed to detect the expression of Cleaved-caspase3 protein; Transwell method was used to detect cell migration, and real-time quantitative polymerase chain reaction (RT-qPCR) to determine the expression of circ-RPL15 and miR-489-3p. The dual luciferase report experiment analyzed the targeted regulation of miR-489-3p by circ-RPL15. Results: Compared with the control group, after different concentrations (25, 50 and 75 μg/mL) of kaempferide acted on lung cancer cell A549, cell viability, clone formation number, migration number and circ-RPL15 expression decreased, while apoptosis rate, Cleaved-caspase3 protein and miR-489-3p expression increased (P<0.05), and all were concentration-dependent. Silencing circ-RPL15 enhanced the expression of miR-489-3p, apoptosis rate and Cleaved-caspase3 protein expression of lung cancer cell A549, and weakened cell viability, the number of clone formation, and the number of migration (P<0.05). circ-RPL15 targets miR-489-3p. Overexpression of circ-RPL15 reversed the proliferation, apoptosis and migration of lung cancer cells that were affected by kaempferide (P<0.05). Conclusion: Kaempferide inhibits the proliferation and migration of lung cancer cells and induces their apoptosis by down-regulating circ-RPL15 and targeting miR-489-3p.
