JIN Lida,WANG Liangrong,XIONG Xiangqing, et al. Effect of Shenmai injection on inflammatory response of rats with ventilator-induced lung injury[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(11): 810-.
Abstract：Objective: To investigate the effect of Shenmai injection on inflammatory response of rats with ventilator-induced lung injury (VILI). Methods: Thirty healthy adult male SD rats weighting 300~350 g were randomly divided into 3 groups (n=10 in each): group TV (traditional tidal volume, VT=8 mL/kg), group HV (high tidal volume, VT=40 mL/kg), group HS (pretreated with Shenmai injection 10 mL/kg and high tidal volume 40 mL/kg). After 4-hour mechanical ventilation, arterial blood samples were obtained for blood gas analysis before the animals were sacrificed. The lungs were removed for observation under microscopic examination, and bronchoaveolar lavage fluid (BALF) was collected. W/D lung weight ratio and pulmonary permeability index (PPI) were calculated, also TNF-α, IL-1β, IL-10 in BALF and Na+-K+-ATPase activity in lung tissue were measured. Results: After 4-hour ventilation, compared with group TV, PaO2 was significantly decreased, PPI, W/D and TNF-α/IL-10 ratio was increased, the levels of TNF-α, IL-1β, IL-10 in BALF were increased and Na+-K+-ATPase activity in lung tissue were decreased in group HV (P<0.05). PaO2 was significantly higher, PPI, W/D and TNF-α/IL-10 ratio were decreased, TNF-α, IL-1β in BALF were decreased, the level of IL-10 in BALF and Na+-K+-ATPase activity in lung tissue were higher in group HS as compared to group HV (P<0.05). The lung tissues injury was severe in group HV, which could be obviously alleviated in group HS. Conclusion: Shenmai injection can attenuate ventilation-induced lung injury in rats, whose mechanism may be related to alleviate the inflammatory response and improve lung water metabolism in VILI.
