1.Department of Medical Oncology, the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015, China; 2.The First School of Medicine, Wenzhou Medical University, Wenzhou 325035, China;3.Department of Breast Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015,China
GU Dianna,CHEN Hanbin,QIAN Fangjing, et al. The inhibiting effect of exosomal ciRS-7 derived from cancer associated fibroblasts on glycolysis of pancreatic cancer cells via miR-7/IGF1R axis[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(11): 861-866.
Abstract：Objective: To investigate the effect of exosomal ciRS-7 derived from cancer associated fibroblasts (CAFs) on glycolysis of pancreatic cancer cells. Methods: Exosomes derived from CAFs were extracted by ultracentrifugation, and the real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of exosomal ciRS-7 and the mRNAs of key glycolytic enzymes in pancreatic cancer cells.ciRS-7-WT or ciRS-7-MT was used to verify the relationship between ciRS-7 and miR-7 in pancreatic cancer by Dual Luciferase Reporter Assay. The protein changes of IGF1R/AKT axis in pancreatic cancer were analyzed by Western blot. Results: The expression of ciRS-7 was up-regulated in the exosomes derived from CAFs. The exosomes released by CAFs promoted glycolytic metabolism and proliferation of pancreatic cancer cells. The exosomes released by pancreatic CAFs with ciRS-7 knock-down could inhibit the expression of glycolysis-related genes in pancreatic cancer cells. And ciRS-7 promoted glycolysis in pancreatic cancer cells through modulating the miR7/IGF1R/AKT pathway. Conclusion: Exosomal ciRS-7 was up-regulated in pancreatic CAFs, and the downregulation of ciRS-7 expression in CAFs could suppress the glycolysis metabolism in pancreatic cancer cells.
