Serological characteristics and gene sequence analysis of rare B (A) subgroup
CHEN Jingsi1, LIN Jiajin1,ZHANG Ying1, YOU Wei1, JIANG Minghua2
1.Department of Blood Transfusion, the Second Affiliated Hospital &Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China; 2.Department of Clinical Laboratory, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou 325027, China
CHEN Jingsi,LIN Jiajin,ZHANG Ying, et al. Serological characteristics and gene sequence analysis of rare B (A) subgroup[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(10): 836-840.
Abstract：Objective: To analyze the serological characteristics and gene sequencing results of rare B(A) subgroup. Methods: Eight specimens with inconsistent positive and negative ABO typing or antigen weakening were collected from the Second Affiliated Hospital of Wenzhou Medical University from March 2013 to June 2021. Polymerase chain reaction sequence-specific primer (PCR-SSP) was used to detect ABO genotyping, direct sequencing method for gene sequencing, polybrene method with type A, B, AB and O donors for cross matching.Results: Eight samples showed AweakB type in routine serological results and B(A) type in gene sequencing,including 2 cases of B(A).04/O.01.02, 2 cases of B(A).02/O.01.02, 2 cases of B(A).04/O.01.01, 1 case of B(A).04/A.01.01, and 1 cases of B(A).02/O.01.01. Main side cross matching with O and B donors was negative,so as the secondary side of cross matching with AB donors. Conclusion: The genotype of rare B (A) subgroup was mainly B(A).04 and B(A).02, with its serological characteristics of “A is weak and B is strong”.
