Effect of islet transplantation on diabetic peripheral neuropathy in diabetic rats
CHEN Yanyan1, LI Jia1,XU Ziqiang2, XIA Nian’ge1.
1.Department of Neurology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China; 2.Department of Transplantation, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China
CHEN Yanyan,LI Jia,XU Ziqiang, et al. Effect of islet transplantation on diabetic peripheral neuropathy in diabetic rats[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(10): 806-810.
Abstract：Objective: To investigate the protective effect of islet transplantation on diabetic peripheral neuropathy and to explore its possible mechanism. Methods: The diabetic model was established by intraperitoneal injection of streptozotocin in SD rats. Islet transplantation was performed 12 weeks after the diabetic modeling. At week 16 after the diabetic modeling, HE staining, mRNA expression of tumor necrosis factor α (TNF-α), expression of B-cell lymphoma-2 (BCL2), BCL-2 associated X (bax) and caspase3 were detected in sciatic nerve of each group. Results: After islet transplantation, the peripheral nerve axons and myelin sheath of peripheral nerve were obviously improved. Besides, the withdrawal latency to pain stimuli significantly increased, and the mRNA expression of TNF-α was obviously lower. Our study has shown upregulation of BCL-2 downregulation of BAX and caspase3 four weeks after islet transplantation, compared with the diabetic group. Conclusion: Islet transplantation can significantly improve the peripheral neuropathy in diabetic rats, probably through the downregulation of TNF-α, via BCL-2/BAX, caspase3 mediated pathways.
