ZHAO Jing,LI Meng,YE Cheng, et al. The mechanism of lncRNA TTN-AS1 in regulating gastric cancer cell migration, invasion and EMT by targeting miR-204-3p[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(10): 782-786，792.
Abstract：Objective: To explore the role of lncRNA TTN-AS1 in regulation the migration, invasion and epithelial-mesenchymal transition (EMT) of gastric cancer cells by targeting miR-204-3p. Methods: qRT-PCR method was used to detect the expression of TTN-AS1 and miR-204-3p in gastric cancer tissues and adjacent tissues. In vitro culture of human gastric cancer cell AGS, si-NC, si-TTN-AS1, miR-NC, miR-204-3p mimics, si-TTN-AS1 and anti-miR-NC, si-TTN-AS1 and anti- miR-204-3p were transfected into AGS cells. The qRT-PCR method was used to detect the expression of TTN-AS1 and miR-204-3p in AGS cells. The Transwell chamber assay was employed to monitor migration and invasion capabilities. The dual luciferase reporter experiment was used to detect the targeting relationship of TTN-AS1 and miR-204-3p. Results: The expression level of TTNAS1 in gastric cancer tissues was increased by about 2.90 times (P<0.05), and the expression level of miR-204-3p was decreased by about 0.57 times compared with adjacent tissues (P<0.05). Transfection of si-TTN-AS1 or transfection of miR-204-3p mimics could significantly reduce the number of migrating and invading cells (P<0.05). TTN-AS1 could target miR-204-3p, which was confirmed by the dual luciferase report experiment.Co-transfection of si-TTN-AS1 and anti-miR-204-3p could significantly increase the number of migrating and invading cells (P<0.05). Conclusion: The inhibition of TTN-AS1 expression could inhibit gastric cancer cell migration, invasion and EMT transformation by up-regulating the expression of miR-204-3p.
