XU Qiulian,WANG Jianfang,XU Heng, et al. Autophagy of placental cells in the pathogenesis of preeclampsia rats and the interventional effect of baicalin[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(9): 755-758.
Abstract：Objective: To discover the role of autophagy of placental cells in the occurrence and development of rat preeclampsia and the interventional effect of baicalin on it. Methods: Totally 48 pregnant Wistar rats were randomly divided into four groups, with 12 in each. Rats in normal pregnancy group were administered with a subcutaneous injection of 1.5 mL normal sodium from Day 13 of pregnancy until Day 21. Rats in preeclampsia group were given a subcutaneous injection of Nitro L Arginine Methyl Ester at 100 mg/kg per day from Day 13 of pregnancy until Day 21. Rats in baicalin group and autophagy inhibition group were treated the same as in preeclampsia group but simultaneously given intraperitoneally injection with baicalin at 50 mg/kg and 3-methyladenine respectively at 15 mg/kg per day from Day 16 of pregnancy until Day 21. At pregnant day 21, the rat tail artery blood pressure and 24 h urine protein level were measured and Western blot was used to determine the expression level of Beclin1, LC3-II and P62 proteins in placental cells. Results: Compared with normal pregnancy group, the expression level of Beclin 1 and LC3-II proteins in placental cells of rats was significantly elevated in preeclampsia group (P<0.05), and tail arterial systolic and diastolic blood pressures and 24 h urine protein level were substantially raised (P<0.05), while expression level of P62 protein was markedly decreased (P<0.05);when compared with preeclampsia group, the expression level of Beclin 1 and LC3-II proteins in placental cells of rats were significantly declined in baicalin group and autophagy inhibition group (P<0.05), and tail arterial systolic and diastolic blood pressures and 24 h urine protein level were substantially decreased (P<0.05), while the expression level of P62 protein was markedly increased (P<0.05). Conclusion: Autophagy of placental cells may be implicated in the occurrence and development of rat preeclampsia, and intraperitoneal injection of baicalin can obviously depress autophagy of placental cells of preeclampsia rats, subsequently exerting its therapeutic effect on preeclampsia.
