The protective effect of pterostilbene on rat diaphragm during sepsis via PI3K-Akt pathway
ZENG Haiping1, WU Dongfang2, HU Yunshuang1
1.Department of Laboratory Medicine, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Wenzhou 325000, China; 2.School of Laboratory Medicine and Life Science, Wenzhou Medical University, Whenzou 325035, China
ZENG Haiping,WU Dongfang,HU Yunshuang. The protective effect of pterostilbene on rat diaphragm during sepsis via PI3K-Akt pathway[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(9): 746-750,754.
Abstract：Objective: To explore the protective effect of pterostilbene (PTS) on sepsis-induced diaphragm injury and its possible mechanism in a rat model. Methods: Sepsis model was induced by cecal ligation and puncture (CLP) in rats. The rats, randomly divided into three groups: sham group, CLP group and PTS groups(25 mg/kg), were killed after CLP for 24 h. Diaphragm tissues were collected for pathological analysis and diaphragm Damage Score (DDS) detection. The apoptosis of diaphragm tissues was evaluated using TUNEL assay. The content of IL-1β, TNF-α, IL-6, AST, LDH, CK and MPO in diaphragm specimen was measured by ELISA assay. Western blot was used to explore the level of p-Akt and p-PI3K in diaphragm tissues. Results:Compared with sham group, the DDS score, apoptotic rate and the content of IL-1β, TNF-α, IL-6, AST, LDH, CK and MPO in CLP group were all promoted markedly. The level of p-Akt and p-PI3K was reduced during sepsis.However, the DDS score, apoptotic rate and the content of IL-1β, TNF-α, IL-6, AST, LDH, CK and MPO in CLP group were all markedly decreased by the administration of PTS, while the expression of p-Akt and p-PI3K was both increased after PTS treatment. Conclusion: PTS plays a protective role in diaphragm via PI3K/Akt pathway in a CLP-induced sepsis rat model, which provides a candidate for drug therapy of sepsis-induced diaphragm
injury.
