The therapeutic effect of Kangfuxin liquid on ulcerative colitis mice
JIN Xumin1, 2, SUN Jie2, XUE Pengpeng2, DONG Weijie3, TAO Xinjie2, XU Helin2
1.Animal Laboratory Centre, Wenzhou Medical University,Wenzhou 325035, China; 2.School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035,China; 3.The First School of Medicine, Wenzhou Medical University, Wenzhou 325035, China
JIN Xumin,SUN Jie,XUE Pengpeng, et al. The therapeutic effect of Kangfuxin liquid on ulcerative colitis mice[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(9): 699-704,712.
Abstract：Objective: To prepare Kangfuxin liquid (KFX) and Dexamethasone (DXM) enema and to evaluate its therapeutic effect on DSS induced ulcerative colitis mice. Methods: The dextran sulfate induced ulcerative colitis mice model was established and the model mice were divided into three groups: Saline group,DXM group and KFX group. The therapeutic effect of these treatment on UC mice was evaluated from aspects of symptom relief, spleen swelling, colon length, and MPO level of colon tissue. Besides, the morphology and mucosal barrier of colon were evaluated by HE staining, IHC staining and immunofluorescence staining respectively. Results: After DXM or KFX treatment, DSS induced UC mice had significantly reduced diarrhea,hematochezia, spleen swelling, colon swelling, colon length growth and MPO level in comparison with Saline group (P<0.05). Moreover, HE staining showed more complete mucosa epithelium layer, crypt, muscle layer mucosa and better-repaired submucosa after KFX treatment in comparison with DXM. No swelling or inflammatory cell infiltration in each layer of colon was observed in KFX group, indicating the effective morphological repair of the damaged colon. IHC staining showed that the secretion of inflammatory factors in KFX group was significantly reduced, compared with DXM group (P<0.05), indicating that colon tissue was effectively repaired. IF staining showed that the secretion of tight junction protein in KFX group was significantly increased (P<0.05), indicating that the damaged colonic mucosal barrier was effectively repaired. Conclusion:KFX oral liquid can effectively relieve UC symptoms and repair the damaged colon.
