The effect of lncRNA TTN-AS1 targeted inhibition of miR-1271 expression on the proliferation, invasion and apoptosis of lung cancer cells and its mechanism
CHEN Zhe1, WANG Qinning1, HUANG Lujie1, SHI Xinbao1, HU Jing2
1.Department of Cardiothoracic Surgery, Ningbo Medical Center Lihuili Hospital, Ningbo 315040; 2.Department of Radiotherapy, Ningbo Medical Center Lihuili Hospital, Ningbo 315040, China
CHEN Zhe,WANG Qinning,HUANG Lujie, et al. The effect of lncRNA TTN-AS1 targeted inhibition of miR-1271 expression on the proliferation, invasion and apoptosis of lung cancer cells and its mechanism[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(8): 632-638.
Abstract：Objective: To investigate the effect of lncRNA TTN-AS1 on the proliferation, invasion and apoptosis of lung cancer cells by regulating miR-1271 expression. Methods: Human lung cancer A549 cells were transfected with TTN-AS1 siRNA or miR-1271 mimics. QRT-PCR was used to detect the expression of TTNAS1 and miR-1271, CCK-8 test to detect cell proliferation, Transwell test to detect cell invasion, flow cytometry to detect cell apoptosis and Western blot to detect the expression of PI3K, p-AKT, PCNA, E-cadherin and cleaved caspase 3. miR-1271 inhibitors and TTN-AS1 siRNA were co-transfected and the effect of miR-1271 inhibition on the proliferation, invasion and apoptosis of A549 cells induced by TTN-AS1 was observed. The dual luciferase reporter gene experiment was performed to detect the targeting effect of TTN-AS1/miR-1271 and miR-1271/PDK1. The effect of over-expression or inhibition of TTN-AS1/miR-1271 on PDK1 expression was analyzed by Western blot. Results: Inhibition of TTN-AS1 and over-expression of miR-1271 could significantly inhibit the proliferation and invasion of A549 cells, promote apoptosis, down-regulate the expression of PI3K, p-AKT and PCNA, and up-regulate the expression of E-cadherin and cleaved caspase 3 (P<0.05). Inhibition of miR-1271 could reverse the inhibitory effect of TTN-AS1 on the proliferation, invasion of A549 cells and the promotion of apoptosis as well. Both TTN-AS1 and PDK1 had a targeted regulatory relationship with miR-1271. Conclusion:lncRNA TTN-AS1 can inhibit the proliferation and invasion of A549 cells and promote apoptosis by targeting miR-1271/PDK1 and inhibiting PI3K/AKT signaling pathway.
