The relationship between placental lncRNA H19/miR-675/PPARα and non-gestational diabetes mellitus macrosomia
DING Miaomiao1, NI Lifang1, ZHENG Tian1, YU Qiuyan1, WANG Yuhuan2, YANG Xinjun1
1.Department of Preventive Medicine, School of Public Health and Management, Wenzhou Medical University,Wenzhou 325035, China; 2.Department of Obstetrics, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China
DING Miaomiao,NI Lifang,ZHENG Tian, et al. The relationship between placental lncRNA H19/miR-675/PPARα and non-gestational diabetes mellitus macrosomia[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(8): 603-308.
Abstract：Objective: To investigate the relationship between placental lncRNA H19/miR-675/PPARα and non-gestational diabetes mellitus (GDM) macrosomia. Methods: A nest-case control study was performed. Full term neonates with birth weight ≥4 000 g in Obstetrics Department of the Second Affiliated Hospital of Wenzhou Medical University from 2014 to 2016 were collected continuously as the macrosomia group, and normal birth weight neonates between 2 500 and 3 999 g were recruited within 3 days from the birth of macrosomia as the control group. Demographic data and placental tissue samples of the two groups were collected. The mRNA expression
level of placental lncRNA H19, miR-675 and PPARα was detected by qPCR. Multivariable logistic regression was used to analyze the relationship between placental lncRNA H19, miR-675, PPARα expression and non-GDM macrosomia. The regulatory effect of lncRNA H19/miR-675 on PPARα was further verified in human chorionic trophoblast cells (HTR-8/SVneo). Results: Non-GDM macrosomia group had lower expression level of placental lncRNA H19 and miR-675 but higher mRNA expression level of PPARα than the control group (P<0.05). Logistic regression analysis showed that low lncRNA H19 and miR-675 and high PPARα expression in placenta increased the risk of non-GDM macrosomia (lncRNA H19: OR=3.69, 95%CI=1.28-10.65; miR-675: OR=3.32, 95%CI=1.20-9.19; PPARα: OR=4.71, 95%CI=1.63-13.57). After the knowckdown of lncRNA H19 in HTR-8/SVneo cells, miR-675 was decreased and the mRNA and protein expression of PPARα was upregulated. However, when lncRNA H19 was knocked down and miR-675 was overexpressed at the same time, the upregulation of mRNA and protein expression of PPARα was suppressed. Conclusion: Low expression of placental lncRNA H19/miR-675 can increase the risk of non-GDM macrosomia and high expression of placental PPARα, which may be partly regulated by lncRNA H19/miR-675, can increase the risk of non-GDM macrosomia.
