The protective effect of estradiol on cardiac function in ischemia reperfusion injury via MIF-AMPK signal pathway
QIAN Lu1, LI Zhuoyuan2, ZHANG Suqin1
1.Department of Cardiology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China; 2.ECG Room, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China
QIAN Lu,LI Zhuoyuan,ZHANG Suqin. The protective effect of estradiol on cardiac function in ischemia reperfusion injury via MIF-AMPK signal pathway[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(7): 548-553.
Abstract：Objective: To investigate the protective effect of estradiol on myocardial ischemia-reperfusion injury in mice and its possible mechanism. Methods: B-ultrasonography and myocardial TTC staining were used to study the effect of estradiol on the acute stage of myocardial ischemia-reperfusion model in mice, and protein western blot was used to detect the effect of estradiol on macrophage migration inhibitory factor (MIF) and downstream AMPK and Acetyl CoA carboxylase (ACC) phosphorylation. Results: The left ventricular ejection fraction and short-axis shortening rate of mice after myocardial ischemia-reperfusion injury under estradiol were improved, compared with other groups (P<0.05). MIF level and AMPK and ACC phosphorylation were increased in ischemia-reperfusion myocardium after estradiol treatment (P<0.05). Conclusion: Estradiol may play a protective role in the acute stage of myocardial ischemia-reperfusion injury in mice through the MIF-AMPK signaling pathway.
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