A comparative study on the two methods of paraffin-embedded renal biopsy for transmission electron microscopy
SHEN Lijun1, LI Duo2, FANG Zhouxi1, CHEN Chaosheng2
1.College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, China; 2.Department of Nephrology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China
SHEN Lijun,LI Duo,FANG Zhouxi, et al. A comparative study on the two methods of paraffin-embedded renal biopsy for transmission electron microscopy[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(5): 413-417.
Abstract：Objective: To compare the application value of two different paraffin-embedded kidney biopsy specimens transferred to electron microscopy in renal pathological diagnosis. Methods: A total of 21 patients,who underwent pathological diagnosis of renal biopsy in the Department of Nephrology of the First Affiliated Hospital of Wenzhou Medical University from May 2018 to September 2019, were enrolled in the study. The two methods of transferring paraffin-embedded tissues into transmission electron microscopy (TEM) samples were compared in aspects of characteristic ultra-structures and the thickness of glomerular basement membranes.Results: It took 18 hours, less than the time (33 h) for the normal way of dewaxing and rehydrating, to process paraffin-embedded renal biopsy samples with fast dewaxing and fixation procedure. Besides, the fast dewaxing and fixation method was more efficient in the preservation of some characteristic ultra-microstructures than the normal way of dewaxing and rehydrating. Both methods created an illusion of getting thinner in glomerular basement membrane (P=0.043, P<0.001), and there was no difference between the two transferring groups (P=0.210). Conclusion: Both methods can be used for clinical diagnosis. However, the fast dewaxing and fixation method is more suitable for clinical pathological diagnosis than the rehydrating way in that the former is better in preserving ultrastructure and short in processing time.
