ZHU Huan,XIE Bingxiang,ZHANG Chongsheng, et al. Quality control of Bletilla striata based on chemometrics method and HPLC fingerprint[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(5): 389-393.
Abstract：Objective: To make an routine inspection of Bletilla striata pieces decoction according to the Chinese Pharmacopoeia and to evaluate its quality by means of HPLC fingerprint, cluster analysis and multivariate statistical analysis. Methods: Chemical properties, including moisture, total ash and residues of sulfur dioxide, of the Bletilla striata pieces decoction were investigated. HPLC was used to determine the fingerprint chromatogram: Thermo Hypersil Gold Aq (4.6 nm×250 mm, 5 μm) column with gradient elution of acetonitrile and 0.05% phosphate acid, the detection wavelength was set at 340 nm, cluster analysis and partial least squares discriminant analysis (PLS-DA) were performed using SPSS and Simca-P software. Results: Six common peaks were found in the HPLC fingerprints of 36 sample batches from different habitats. Cluster analysis and PLS-DA model [R2X (cum)=0.63, R2Y (cum)=0.71, Q2=0.59] showed clearly discriminated samples, with the NO.5 chromatographic peak being the major contributor to the differential profiles. Conclusion: Difference in chemical composition of Bletilla striata exists on the market and the study results can be the reference for quality control of decoction pieces of Bletilla striata.
