The role of macrophages’ gene expression in the pathology of middle ear cholesteatoma
FANG Lian1, CHEN Lin2, LIN Bi1
1.Department of Otolaryngology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China; 2.Department of Pathology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China
FANG Lian,CHEN Lin,LIN Bi. The role of macrophages’ gene expression in the pathology of middle ear cholesteatoma[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(5): 352-357,363.
Abstract：Objective: To analyze gene expression profiles of macrophages in middle ear and explore the molecular mechanism in the progression of middle ear cholesteatoma based on differential genes. Methods:Cholesteatoma perimatrix or granulation tissue was collected from 53 patients with middle ear cholesteatoma or chronic otitis media, who underwent mastoidectomy in ENT department from January 2018 to December 2018.Three patients with cholesteatoma were selected randomly as an experimental group and three patients without cholesteatoma as a control group. Gene expression profiles of two groups were compared and the underlying function of differentially expressed genes predicted by GO and KEGG enrichment analysis. The underlying trend of top genes was identified by key genetic analysis. Results: The current study showed that 124 out of 7 060 genes were significantly up-regulated (P<0.05) when compared with the patients without cholesteatoma. Gene enrichment analysis showed that these altered genes were involved in multiple signaling pathways associated with inflammatory and immune response. Keratin-13 and MMPs genes that affected the abnormal proliferation and bone destruction were significantly up-regulated in cholesteatoma. Conclusion: Our results suggest that these
differential genes of macrophages are involved in multiple signaling pathways associated with inflammation and immunity. Keratin-13 and MMPs are key genes that affect cellular proliferation and bone destruction and may play a critical role in the progression of cholesteatoma.
