Expression profile of methylation regulated lncRNA and its significance in breast cancer
HU Qian1, LAN Xiaoshan1, LIN Yingxin1, CHEN Xiuyun1, DING Linxiaoxiao2, PANG Danmei1
1.Department of Oncology, the First Hospital of Foshan, Foshan 528000, China; 2.Breast Cancer Center, Sun Yat-Sen Memorial Hospital, Guangzhou 510120, China
HU Qian,LAN Xiaoshan,LIN Yingxin, et al. Expression profile of methylation regulated lncRNA and its significance in breast cancer[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(4): 271-275,281.
Abstract：Objective: To study the differential expression of lncRNA between demethylated breast cancer cells and their parental lines, and to screen lncRNA from differential expression profile, so as to explore the role of lncRNA regulated by DNA promoter methylation in the occurrence and development of breast cancer. Methods: After demethylation of breast cancer BT474 cell line, the expression profiles of lncRNA in demethylation group and parental group were detected by high-throughput lncRNA microarray technology, and the expression of lncRNA in breast cancer cell line BT474, MB231, BT549 and cancer tissue were detected by qRT-PCR technology.
Meanwhile, the gene methylation sites were searched by bisulfite sequencing PCR. Results: LncRNA expression profiles of demethylated and parental cells have significantly changed. The results of quantitative RT-PCR and microarray were basically consistent. Quantitative RT-PCR showed that the expression of lncRNA uc003lxs and uc002btf in breast cancer tissues and breast cancer cell lines were higher than that in normal breast epithelial cell lines (P<0.01), and the expression of lncRNA uc003lxs and uc002btf in breast cancer tissues was higher than that in adjacent tissues (P<0.01). Compared with normal breast epithelial cell lines, the DNA methylation levels of lncRNA uc003lxs and uc002btf in breast cancer tissues and breast cancer cell lines were significantly decreased by BSP method (P<0.01); compared with adjacent tissues, the DNA methylation levels of lncRNA uc003lxs and uc002btf in breast cancer tissues were significantly decreased (P<0.01). Conclusion: The expression of lncRNA in breast cancer cell lines after demethylation treatment has been found to be significantly changed, and lncRNA uc003lxs and uc002btf have been screened out, which lays a preliminary research foundation for the study of methylation regulated lncRNA in the regulation of the occurrence and development of breast cancer.
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