ZHANG Jiafeng,ZHAO Hui,HE Ping, et al. The effect of Quercetin on human nasopharyngeal cancer cell growth and AKT/mTOR expression[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(4): 266-270.
Abstract：Objective: To investigate the effect of quercetin on cell growth and AKT/mTOR pathway expression in human nasopharyngeal carcinoma 5-8F cells. Methods: 5-8F cells were treated with different concentrations of quercetin (20, 40 and 60 μmol/L) for 24 h, 48 h and 72 h before MTT assay was performed to detect the cell growth. Quantitative PCR and Western blot were used to detect the expression levels of AKT and mTOR. A nasopharyngeal carcinoma mouse model was established through subcutaneously inoculated with 5-8F cells in nude mice. The mouse model was randomly divided into the control group and quercetin-treated group. Tumor volume was measured every 7 days. Western blot and immunohistochemistry were used for detection of AKT and mTOR protein levels in tissues. Results: 5-8F cells growth was significantly inhibited in the quercetin-treated group (P<0.05), compared with the control group. Quercetin reduced the expression levels of AKT and mTOR in 5-8F cells (P<0.05). In in vivo experiments, the tumor volume in nude mice in the quercetin-treated group was significantly inhibited (P<0.05), and the expression of AKT and mTOR in tumor tissues was also significantly decreased (P<0.05). Conclusion: Quercetin inhibits cell growth and AKT/mTOR pathway expression in nasopharyngeal carcinoma 5-8F cells
参考文献：
[1] SABA N F, SALAMA J K, BEITLER J J, et al. ACR Appropriateness criteria® for nasopharyngeal carcinoma[J]. Head Neck, 2016, 38(7): 979-986.
[2] MOHAMMED M A, ABD GHANI M K, HAMED R I, et al. Review on nasopharyngeal carcinoma: Concepts, methods of analysis, segmentation, classification, prediction and impact: A review of the research literature[J]. J Comput Sci, 2017, 21: 283-298.
[3] WU L, LI C, PAN L. Nasopharyngeal carcinoma: A review of current updates[J]. Exp Ther Med, 2018, 15(4): 3687- 3692.
[4] AL-SARRAF M, LEBLANC M, GIRI P G, et al. Chemoradiotherapy versus radiotherapy in patients with advanced nasopharyngeal cancer: phase III randomized Intergroup study 0099[J]. J Clin Oncol, 1998, 16(4): 1310- 1317.
[5] DAI W, ZHENG H, CHEUNG A K, et al. Genetic and epigenetic landscape of nasopharyngeal carcinoma[J]. Chin Clin Oncol, 2016, 5(2): 16.
[6] ZHANG M, SWARTS S G, YIN L, et al. Antioxidant properties of quercetin[J]. Adv Exp Med Biol, 2011, 701: 283-289.
[7] RAUF A, IMRAN M, KHAN I A, et al. Anticancer potential of quercetin: A comprehensive review[J]. Phytother Res, 2018, 32(11): 2109-2130.
[8] WANG W, SUN C, MAO L, et al. The biological activities, chemical stability, metabolism and delivery systems of quercetin: A review[J]. Trends Food Sci Technol, 2016, 56: 21-38.
[9] ZHANG F, CUI Y, CAO P. Effect of quercetin on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells[J]. J Huazhong Univ Sci Technolog Med Sci, 2008, 28(3): 369-372.
[10] 顾生玖, 杨娜, 朱开梅. 槲皮素抑制人鼻咽癌CNE2细胞生长并诱导其凋亡的研究[J]. 中国实验方剂学杂志, 2011, 17(13): 192-194.
[11] CHO W K, OH D, LEE E, et al. Feasibility of selective neck irradiation with lower elective radiation dose in treating nasopharynx cancer patients[J].Cancer Res Treat, 2019, 51(2): 603-610.
[12] KONG F, ZHOU J, DU C, et al. Long-term survival and late complications of intensity-modulated radiotherapy for recurrent nasopharyngeal carcinoma[J]. BMC Cancer, 2018, 18(1): 1139.
[13] KIM C, KIM B. Anti-cancer natural products and their bioactive compounds inducing ER stress-mediated apoptosis: A review[J]. Nutrients, 2018, 10(8): 1021.
[14] 秦肖菲, 陈永新. 地榆皂苷抗肿瘤机制及药物递送研究进展[J]. 中国药剂学杂志, 2020, 18(3): 180-186.
[15] ZHU A, ZHOU H, XIA J Z, et al. Ziyuglycoside II-induced apoptosis in human gastric carcinoma BGC-823 cells by regulating Bax/Bcl-2 expression and activating caspase-3 pathway[J]. Braz J Med Biol Res, 2013, 46(8): 670-675.
[16] KIM D H, KHAN H, ULLAH H, et al. MicroRNA targeting by quercetin in cancer treatment and chemoprotection[J]. Pharmacol Res, 2019, 147: 104346.
[17] ZIZKOVA P, STEFEK M, RACKOVA L, et al. Novel quercetin derivatives: from redox properties to promising treatment of oxidative stress related diseases[J]. Chem Biol Interact, 2017, 265: 36-46.
[18] SU Q, PENG M, ZHANG Y, et al. Quercetin induces bladder cancer cells apoptosis by activation of AMPK signaling pathway[J]. Am J Cancer Res, 2016, 6(2): 498-508.
[19] WARD A B, MIR H, KAPUR N, et al. Quercetin inhibits prostate cancer by attenuating cell survival and inhibiting anti-apoptotic pathways[J]. World J Surg Oncol, 2018, 16(1): 108.
[20] DIENSTMANN R, RODON J, SERRA V, et al. Picking the point of inhibition: a comparative review of PI3K/AKT/ mTOR pathway inhibitors[J]. Mol Cancer Ther, 2014, 13(5): 1021-1031.
[21] JIA L, HUANG S, YIN X, et al. Quercetin suppresses the mobility of breast cancer by suppressing glycolysis through Akt-mTOR pathway mediated autophagy induction[J]. Life Sci, 2018, 208: 123-130.
[22] GRANATO M, RIZZELLO C, GILARDINI MONTANI M S, et al. Quercetin induces apoptosis and autophagy in primary effusion lymphoma cells by inhibiting PI3K/AKT/mTOR and STAT3 signaling pathways[J]. J Nutr Biochem, 2017, 41: 124-136.
[23] WANG W, WEN Q, XU L, et al. Activation of Akt/mTOR pathway is associated with poor prognosis of nasopharyngeal carcinoma[J]. PLoS One, 2014, 9(8): e106098.
[24] LIU Y, LIU Q, CHEN S, et al. APLNR is involved in ATRAinduced growth inhibition of nasopharyngeal carcinoma and may suppress EMT through PI3K-Akt-mTOR signaling[J]. FASEB J, 2019, 33(11): 11959-11972.
[25] HUANG W, ZENG C, LIU J, et al. Sodium butyrate induces autophagic apoptosis of nasopharyngeal carcinoma cells by inhibiting AKT/mTOR signaling[J]. Biochem Biophys Res Commun, 2019, 514(1): 64-70.
