Differential expression profiles and functional prediction of circRNAs in ox-LDL stimulated macrophages
YE Bozhi1, LIANG Xiaohe1, ZHAO Yihan1, XUE Qize2, HUANG Zhouqing1, CAI Xueli1
1.Department of Cardiology, the Key Lab of Cardiovascular Disease of Wenzhou, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China; 2.The Second School of Medicine, Wenzhou Medical University. Wenzhou 325035, China
YE Bozhi,LIANG Xiaohe,ZHAO Yihan, et al. Differential expression profiles and functional prediction of circRNAs in ox-LDL stimulated macrophages[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(3): 203-208.
Abstract：Objective: To investigate the differential expression profiles of circRNAs in ox-LDL-stimulated macrophages and predict its functions. Methods: Human monocyte cell line THP-1 cells were pretreated with PMA for 48 h and followed by ox-LDL (50 µg/mL) for 24 h to establish cell model. Microarray analysis was performed to investigate the differential expression profiles of circRNAs in macrophages with or without ox-LDL stimulation (as a control). qRT-PCR was utilized to verify the content variation of circRNAs in macrophages with ox-LDL stimulation. CircInteractome, TargetScan and miRecords websites were used to predict potential target genes. Results: There were 5 upregulated circRNAs and 2 downregulated circRNAs in ox-LDL-stimulated macrophages group, compared with the control group in the present study. We also verified that there were 4 circRNAs upregulated and 2 circRNAs downregulated in macrophages with ox-LDL stimulation by qRT-PCR. Bioinformatics analysis speculated that circRNA_0007478 could affect the expression of downstream proteins by binding to miR-765. Conclusion: There exist differential expressions of circRNAs in ox-LDL-stimulated macrophages. CircRNA_0007478 is predicted to be involved in the atherosclerosis process by binding to miRNA-765 to regulate downstream genes.
