SGLT1 mediates high glucose-induced cardiac fibroblast activation
WANG Ping1, MENG Liping1, LIU Longbin2, GUO Hangyuan 1, 3, 4
1.Department of Cardiology, Shaoxing People’s Hospital, Shaoxing Hospital of Zhejiang University, Shaoxing 312000, China; 2.Department of Cardiology, Affiliated Hospital of Shaoxing College of Arts and Sciences, Shaoxing Municipal Hospital, Shaoxing 312000, China; 3.Graduate School of Wenzhou Medical University, Wenzhou 325035, China; 4.Shaoxing Arts and Science College of Medicine, Shaoxing 312000, China
WANG Ping,MENG Liping,LIU Longbin, et al. SGLT1 mediates high glucose-induced cardiac fibroblast activation[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(3): 173-180.
Abstract：Objective: To investigate whether high glucose can activate cardiac fibroblasts and the role of SGLT1 in the activation. Methods: The 4th-7th generations of cardiac fibroblasts were used in the experiment. Incubated with different concentration of glucose, the cell was morphologically observed under microscope; the cell proliferation capacity was measured by MTT and EdU assay, the cell migration ability was measured by wound healing test and Transwell test; the expression of SMα, Tcf21 and SGLT1 was tested by immunofluorescence, qRT-PCR and Western blot. Then si-SGLT1 was used to knock out SGLT1 in the cells, and the indicators were tested again by each method. Results: Compared with the control group, cells in the high glucose group were round in morphology, and increased in migration, proliferation ability and increased expression of SMα, Tcf21 and SGLT1 (P<0.05). After transfection with si-SGLT1, SGLT1 expression in the cells was decreased (P<0.05). Meanwhile, the migration, proliferation ability and the expression of SMα, Tcf21 were also decreased (P<0.05). Conclusion: High glucose activated cardiac fibroblasts by upregulating SGLT1.
