Effect of HMGA gene silencing on the growth and metastasis of non-small cell lung cancer A549 cells
LI Binwu1, YE Zhenyue2, YANG Sijia2
1.Department of Pneumology, Ningbo Fenghua District Hospital of Traditional Chinese Medicine, Ningbo 315500, China; 2.Department of Pneumology, Hwa Mei Hospitial,University of Chinese Academy of Sciences, Ningbo 315010, China
LI Binwu,YE Zhenyue,YANG Sijia. Effect of HMGA gene silencing on the growth and metastasis of non-small cell lung cancer A549 cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(2): 105-110,117.
Abstract：Objective: To investigate the effect of HMGA1 and HMGA2 gene silencing on the malignant proliferation and metastasis of human non-small cell lung cancer A549 cells and the corresponding mechanism.Methods: The proliferation capacity of A549 cells was analyzed by MTT assay after silencing HMGA1 and HMGA2 gene. The effect of HMGA1 and HMGA2 gene silencing on the apoptosis of A549 cells were determined by flow cytometry. Scratch assay was used to analyze the migration ability of A549 cells after silencing HMGA1and HMGA2 genes. Q-PCR and western blot were used to detect the expression of apoptosis and metastasis related factors in mRNA and protein level after HMGA1 and HMGA2 gene silencing. Results: Silencing HMGA1 and HMGA2 genes with siRNA-HMGA1 and siRNA-HMGA2 for 48 h, A549 cell viability decreased (P<0.05).The results of Q-PCR showed that siRNA-HMGA1 inhibited the expression of apoptosis related genes FOXM1 and c-Myc (P<0.05). The effect of siRNA-HMGA1 on the expression of genes related to invasion (E-Cadherin,N-Cadherin and Vimentin) had no statistical difference (P>0.05). The results of western blot confirmed that siRNA-HMGA1 impaired the expression of FOXM1 and c-Myc in A549 cells (P<0.05). Similarly, siRNAHMGA2 inhibited the expression of FOXM1 and c-Myc (P<0.05). Meanwhile, siRNA-HMGA2 caused the expression of E-Cadherin (P<0.05), and inhibited the expression of N-Cadherin and Vimentin (P<0.05). Western blot confirmed the results of Q-PCR, where siRNA-HMGA2 impaired the expression of FOXM1 and c-Myc (P<0.05), increased the expression of E-Cadherin (P<0.05), and blocked the expression of N-Cadherin and Vimentin (P<0.05). Conclusion: Silencing HMGA1 and HMGA2 gene induced the apoptosis of A549 cells through down-regulating the expression of FOXM1 and c-Myc. Silencing HMGA2 gene inhibited the metastasis of A549 cells though down-regulation of N-Cadherin and Vimentin, and upregulating of E-Cadherin.
