ZHU Ronghe,SUN Yuanyuan,WANG Kai, et al. The involvement of serum extracellular histones in the progression of hypoxic ischemic encephalopathy in newborn rats by promoting apoptosis[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(2): 93-98.
Abstract：Objective: To investigate whether extracellular histones are involved in the injury of neonatal rat hypoxic-ischemic encephalopathy by promoting apoptosis. Methods: Fifty-four 7-day-old SD rats were randomly divided into as sham-operation group, Hypoxic-ischemic brain damage (HIBD) group and treatment group, each group was randomly subdivided into the 6 h, 24 h and 72 h groups (6 rats each subgroup). The HIBD model was established using the improved rice method. Plasma extracellular histones and the brain tissue histopathologic changes and the index of apoptosis were observed 6 hours, 24 hours and 72 hours after HIBD. Results: In the HIBD group, compared with the sham group, the brain water content was increased (P<0.05). HE staining showed the brain tissue structure was damaged and Nidner staining showed the brain tissue loss rate was increased (P<0.05). The plasma extracellular histone content was increased (P<0.05). Tunel staining showed apoptotic cells were increased and expression of apoptotic protein cleaved caspase-3 was increased (P<0.05).Compared with HIBD group, the brain water content was decreased (P<0.05). HE staining showed the brain tissue structure damage was decreased and Nidner staining showed the brain tissue loss rate was decrease (P<0.05).The plasma extracellular histone content was decrease (P<0.05). Tunel staining showed apoptotic cells were decreased and the expression of apoptotic protein cleaved caspase-3 was increased (P<0.05). Conclusion: Serum extracellular histones may be involved in the injury of hypoxic ischemic encephalopathy in newborn rats by promoting apoptosis.
