LAN Xiu,LI Weiwen,SUN Lei, et al. The effect of IGFBP-4 treatment on immunogenic cell death in A549 cells and its mechanism[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(1): 49-52,57.
Abstract：Objective: To investigate the effect of IGFBP-4 treatment on immunogenic cell death in A549 cells and its molecular mechanism. Methods: A549 cells were cultured in vitro and assigned to the control group, IGFBP-4 group, IGFBP-4+NAC group and IGFBP4+si-p38 group, respectively. After 48 hours, MTT was used to detect the survival rate of cells; flow cytometry was used to detect ROS content and calreticulin (CRT); ELISA was used to detect HMGB1 in medium and SOD and MDA in cells; Western blot was used to analyzed the expression of phosphorylated p38 (p-p38) and total p38. Results: Compared with control group, the content of ROS, CRT, HMGB1, MDA, the expression of p-p38 and p38 increased, while the survival rate and content of SOD2 decreased in IGFBP-4 group (P<0.05). Compared with IGFBP-4 group, the content of ROS, CRT, HMGB1, MDA decreased, while the survival rate and content of SOD2 increased in IGFBP4+NAC group and IGFBP4+si-p38 group (P<0.05). Conclusion: IGFBP-4 promoted immunogenic cell death via activating p38 MAPK signaling and inducing oxidative stress in A549 cells.
