Expression of SOAT1 and its clinical significance in hepatocellular carcinoma
CHEN Yao1, 2, YANG Xunjun3, CHEN Hanbin2, CHEN Guorong1, 2
1.Wenzhou Medical University, Wenzhou 325035, China; 2.Department of Pathology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China; 3.Center of Laboratory Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China
CHEN Yao,YANG Xunjun,CHEN Hanbin, et al. Expression of SOAT1 and its clinical significance in hepatocellular carcinoma[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(1): 35-39.
Abstract：Objective: To investigate the expression of sterol O-acyltransferase 1 (SOAT1) in hepatocellular carcinoma (HCC) tissues and tissues adjacent to the cancer, and to explore its correlation with patient’s prognosis and clinicopathological features. Methods: The HCC dataset was collected from the Cancer Genome Atlas (TCGA) database, and download the expression of SOAT1 mRNA and prognostic data. The expression of SOAT1 mRNA in 50 normal liver tissues and 374 HCC tissues was compared to analyze the correlation between expression and prognosis. Collected paraffin tissue specimens of 53 HCC patients from the First Affiliated Hospital of Wenzhou Medical University from May 2018 to October 2019, using immunohistochemical (IHC) methods to detect the protein expression level of SOAT1 in cancer and adjacent tissues, and its correlation with clinical pathology features was analyzed. Results: Data analysis of TCGA database showed that compared with normal tissues adjacent to cancer, SOAT1 expression was significantly increased in HCC (P<0.001). The prognostic survival analysis found that 5-year survival rate of high expression of SOAT1 was lower (P<0.05). IHC results showed that compared with adjacent tissues, the expression of SOAT1 protein in HCC tissues was significantly increased (P<0.05). The expression of SOAT1 protein in cancer tissues was correlated with the clinical stage of the tumor and microvascular tumor thrombosis (P<0.05). Conclusion: The expression of SOAT1 is increased in HCC tissues, and closely related to the clinicopathological characteristics of HCC cancer patients, with its expression highly correlated with the malignancy degree of the tumor.
