LIU Huanxing,CAI Zhongqing,SHEN Xinsheng, et al. Experimental study on the effect of Xuefu Zhuyu Tang on the survival of random skin flaps[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(12): 994-997, 1002.
Abstract：Objective: To invetigate the effect of Xuefu Zhuyu Tang (XZT) on the survival of random skin flaps and to make clear of its appropriate dose. Methods: Totally 72 male SD rats were used to establish a modified McFarlane flap model. Then the animals were randomly divided into 4 groups (n=18): control group with 10 mL·kg-1·d-1 saline by gavage; high-dose XZT group (high-dose group) with 13 g·kg-1·d-1 crude drug by gavage;medium-dose XZT group (medium-dose group) with 6.5 g·kg-1·d-1 crude drug by gavage andlow-dose XZT group (low-dose group) with 3.25 g·kg-1·d-1 crude drug by gavage. Seven days after continuous administration,the survival area of random skin flaps in each group was measured, and the tissue samples were harvested for histological (HE) staining, by which the micro-vessel density (MVD) was evaluated. Western blotting (WB) was used to detect the expression of (vascular endothelial growth factor) VEGF. And the (superoxide dismutase) SOD and (malondialdehyde) MDA content in tissues were evaluated. Results: At 7 days after operation, the survival areas of flaps in the different doses XZT groups were significantly higher than in the control group (P<0.01).HE staining showed an obvious increase in micro-vessel density (P<0.01), and VEGF expression was improved
(P<0.05). Among them, the middle-dose group has the most obvious therapeutic effect. In addition, the content of SOD in the flaps of different dose XZT groups was significantly higher than that in the control group, and MDA was reduced (P<0.05). Conclusion: XZT can promote the survival of rat random flaps, with the most obvious
therapeutic effect shown by the middle-dose group. The underlying mechanism of XZT for the survival of random skin flaps probably lies in that the drug promotes VEGF expression in ischemic tissue, thereby increasing neovascularization in the tissue and promoting microcirculation reconstruction while inhibiting ischemiareperfusion
injury.
