A novel silk fibroin hydrogelcarry cimetidine in the prevention of ethanol-induced gastric ulcer in mice
CHEN Pianpian1, HUANG Weijie1, SHEN Shurong2.
1.TCM Pharmacy, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Wenzhou 325000, China; 2.Department of Oncology, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Wenzhou 325000, China
CHEN Pianpian,HUANG Weijie,SHEN Shurong.. A novel silk fibroin hydrogelcarry cimetidine in the prevention of ethanol-induced gastric ulcer in mice[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(12): 974-979.
Abstract：Objective: To explore the protective effect of novel silk fibroin (SF) hydrogel with cimetidine(CMD) against ethanol-induced acute gastric ulcer in a mice model. Methods: Silk fibroin protein was extracted from the silkworm and was used to prepare cimetidine-silk fibroin hydrogel system. Eight-week-old male C57BL/6 were randomized into four groups of eight animals each:control group, CMD group, CMD-SF solution group and CMD-SF hydrogel group. The microstructure and gelling state of silk fibroin hydrogel were respecctively measured by scanning electron microscope (SEM) and fourier-transform infrared spectrum (FT-IR) analysis. The CMD-SF hydrogel system with sustained cimetidine release in vitro was tested. Additionally, histological sections were stained with haematoxylin and eosin, and Periodic acid-Schiff (PAS) staining. The levels of Superoxide dismutase (SOD) activity as well as malondialdehyde (MDA) were detected in gastric tissue by SOD/MDA assay kit. Immunoblotting and immunohistochemistry were carried out to assess the expression of apoptosis-associated proteins. Results: Silk fibroin hydrogel was successfully prepared based on the results of SEM and FT-IR. CMD showed a sustained release from CMD-SF hydrogel system over 72 h. From the results of HE staining, PAS staining, immunohistochemistry staining and immunoblotting, CMD-SF hydrogel group had more significant effect than the control group on preventing the occurrence of alcohol-induced acute gastric ulcer and reducing gastric mucosal damage. Meanwhile, compared with the control group, SOD level in CMD-SF
hydrogel group increased to (0.61±0.11)U/g prot, and MDA level decreased to (2.42±0.38)nmol/mg prot, which effectively alleviated the oxidative stress response. Conclusion: Using CMD-SF hydrogel system effectively prevented alcohol-induced acute gastric ulcer in a mice model, which could sustain CMD release and prolong
length of stay on the surface of gastric mucosa. Inhibition of oxidative stress response might be a potential mechanism for CMD-SF hydrogel system against tissue damage.
