DONG Zhanglei,LIANG Yuyuan,SU Ying, et al. The effect of glucocorticoid receptor on propofol self-administration of rats and its mechanisms[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(12): 966-969.
Abstract：Objective: To determine whether glucocorticoid receptor involves in propofol self-administration of rats, so as to offer reference for clinical prevention and treatment. Methods: In one experimental group, twenty-four Sprague-Dawley rats were trained to self-administer propofol under a fixed ration (FR1) schedule of reinforcement for 14 d. Then, the rats were treated with saline (control group) and RU486, a GR antagonist (10,20, 40 mg/kg) at 1 h prior to the last training to observe the change of animal behavior. Western blot was used to detect the dopamine DAT level in NAc of propofol dependent. In the other experimental group, twenty-four rats were trained to nose-poke for sucrose pellets under FR1 schedule of reinforcement of a daily 0.5-h session for successive 7 days. Thenthe trained rats received intraperitoneally either saline or a dose of RU486 (10, 20,40 mg/kg) at 1 h prior to the last training to observe the change of animal behavior. Results: RU486 significantly decreased the number of injections and active responses (P<0.01), which was associated with the large decrease in DAT of NAc (P<0.05). However, the food-maintaining sucrose responses under FR1 schedule was not affected by any doses of RU486 tested (P>0.05). Conclusion: The present results indicate that RU486 may counteract the
reinforcing properties of propofol, playing an important role in the incubation of propofol dependent by influencing the dopamine DAT levels.
