丹皮酚对血管性认知障碍小鼠认知功能的保护作用

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (0 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的：探讨丹皮酚对血管性认知障碍（VCI）小鼠认知功能及海马神经元损伤的影响。方法：雄性C57BL/6小鼠60只，随机分为假手术（Sham）组、模型（VCI）组、丹皮酚（Pae）低、中、高剂量治疗组，每组12只。采用双侧颈总动脉结扎（2VO）制备小鼠反复缺血再灌注VCI模型，Morris水迷宫检测小鼠的认知功能，尼氏染色观察小鼠海马神经元的形态学变化，比色法检测海马SOD活性、MDA和NO含量的变化。结果：VCI小鼠认知功能下降（P＜0.01），海马CA1区神经元变性坏死，MDA和NO含量增加，SOD活性降低（P＜0.01）；丹皮酚中、高剂量治疗可显著提高VCI小鼠的学习记忆能力，减少VCI所致的海马CA1区神经元死亡，并降低MDA和NO含量，升高SOD活性（P＜0.05或P＜0.01）。结论：丹皮酚可有效增强VCI小鼠的认知功能，并改善海马神经元病理改变，可能与其抗氧化应激有关。

	服务
	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	王浩1
	耿赵铭2
	呙登俊1
	胡智伟1
	王百辰1

关键词 ：认知障碍, 血管性, 抗氧化, 海马, 丹皮酚, 小鼠

Abstract：Objective: To investigate the effects of paeonol on cognitive dysfunctions and hippocampal neuronal injury of vascular cognitive impairment (VCI) mice. Methods: Sixty C57BL/6 mice were randomly divided into sham opertated group, the VCI group and the paeonol treated groups (low, medium and high dose). The VCI models were dupilicated by the bilateral common carotid artery occlusion (2VO). The cognitive impairments were observed by Morris water maze. The contents of MDA, SOD and NO in hippocampus were determined spectrometrically. The hippocampal neuron injuries in the CA1 region were detected by nissle staining. Results: VCI induced the decreased cognitive function and neuron injury in the hippocampal CA1 region (P<0.01). And MDA and NO contents were significantly increased, whereas SOD activity was dramatically decreased after VCI (P<0.01). Compared with VCI model goup, the medium and high dose of paeonol treatment significantly improved cognitive impairments and reduced pathological changes in hippocampal CA1 region (P<0.05, P<0.01). In additon, the medium and high dose of paeonol treatment markedly improved SOD activity, decreased MDA and NO contents in hippocampus (P<0.05, P<0.01). Conclusion: Paeonol can improve cognitive impairments and neuronal injury in VCI mice, which may be related to the increased antioxidative ability of brain tissue.

Key words： cognitive impairment, vascular antioxidant hippocampus paeonol mice

收稿日期: 2014-04-09

基金资助:浙江省自然科学基金资助项目（Y2110679）。

通讯作者: 胡智伟，主任医师，Email：huzhiwei2008@163.com

作者简介: 王浩（1982-），男，山东枣庄人，主治医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2014/V44/I10/708

[1]Moorhouse P, Rockwood K. Vascular cognitive impairment: current concepts and clinical developments[J]. Lancet Neu-rol, 2008, 7(3): 246-255.
[2]Thal DR, Grinberg LT, Attems J. Vascular dementia: differ-ent forms of vessel disorders contribute to the development of dementia in the elderly brain[J]. Exp Gerontol, 2012, 47(11): 816-824.
[3]Baskys A, Cheng JX. Pharmacological prevention and treat-ment of vascular dementia: approaches and perspectives[J].Exp Gerontol, 2012, 47(11): 887-891.
[4]李瑞奇, 白明, 苗明三. 中药活性成分防治脑缺血特点分析[J]. 中医学报, 2013, 28(8): 1184-1187.
[5]Deng W, Aimone JB, Gage FH. New neurons and new mem-ories: how does adult hippoca-mpal neurogenesis affect learning and memory[J]. Nat Rev Neurosci, 2010, 11(5): 339-350.
[6]Jiwa NS, Garrard P, Hainsworth AH. Experimental models of vascular dementia and vascular cognitive impairment: a systematic review[J]. J Neurochem, 2010, 115(4): 814-828.
[7]曲娴, 李春颖, 苏畅, 等. 碱性成纤维细胞生长因子对血管性痴呆小鼠学习记忆及海马Calbindin-D28K表达的影响[J]. 中华医学杂志, 2010, 90(31): 2219-2222.
[8]胡意员, 周利飞, 何金彩, 等. 长期高脂饮食对小鼠认知功能及情绪的影响[J]. 温州医学院学报, 2013, 43(4): 241-244, 248.
[9]赵惠玲, 丁茂超, 胡斯旺, 等. 针刺对AlCl3致痴呆大鼠空间记忆障碍的改善作用[J]. 温州医学院学报, 2011, 41(1): 10-12.
[10]Gupta S, Sharma B, Singh P, et al. Modulation of transient receptor potential vanilloid subtype 1 (TRPV1) and norepi-nephrine transporters (NET) protect against oxidative stress, cellular injury, and vascular dementia[J]. Curr Neurovasc Res, 2014, 11(2): 94-106.
[11]杨正生, 彭振辉, 姚青海, 等. 丹皮酚的药理作用研究进展[J]. 中国药物与临床, 2011, 11(5): 545-547.
[12]Li H, Xie YH, Yang Q, et al. Cardioprotective effect of pae-onol and danshensu combinati-on on isoproterenol-induced myocardial injury in rats[J]. Plos One, 2012, 7(11): e48872.
[13]Hu S, Shen G, Zhao W, et al. Paeonol, the main active prin-ciples of paeonia moutan, ameliorates alcoholic steatohepa-titis in mice [J]. J Ethnopharmacol, 2010, 128(1): 100-106.
[14]张金艳, 赵乐, 余学钊, 等. 丹皮酚对健康和高血脂大鼠肾动脉和冠状动脉的舒张作用[J]. 中国新药杂志, 2013, 22(20): 2414-2417.
[15]Tseng YT, Hsu YY, Shih YT, et al. Paeonol attenuates mi-croglia-mediated inflammation and oxidative stress-induced neurotoxicity in rat primary microglia and cortical neurons[J]. Shock, 2012, 37(3): 312-318.
[16]杨青, 武继彪, 张岫美. 丹皮酚对大鼠脑缺血再灌注损伤炎性细胞因子的作用[J]. 中国生化药物杂志, 2010, 31(2): 11l-113.
[17]Wu JB, Song NN, Wei XB, et al. Protective effects of pae-onol on cultured rat hippocampal neurons against oxygen-glucose deprivation-induced injury[J]. J Neurol Sci, 2008, 264(1-2): 50-55.
[18]周军, 周丽, 侯德仁, 等. 丹皮酚对Aβ诱导的AD模型大鼠脑细胞凋亡因子的影响及作用机理[J]. 中药材, 2011, 34(5): 758-762.
[19]Liu J, Feng L, Ma D, et al. Neuroprotective effect of pae-onol on cognition deficits of diabetic encephalopathy in streptozotocin-induced diabetic rat[J]. Neurosci Lett, 2013, 549: 63-68.
[20]Zhong SZ, Ge QH, Qu R, et al. Paeonol attenuates neurotox-icity and ameliorates cognitive impairment induced by d-ga-lactose in ICR mice[J]. J Neurol Sci, 2009, 277(1-2): 58-64.