The protective effect of UCF-101 on neurological function of rats with subarachnoid hemorrhage
CHENG Zhenyu1, SHENTU Huasong1, CHEN Yihua1, HE Zhongping2, YU Beiwei3, WANG Ruiquan4, FU Bin1.
1.Department of Neurosurgery, Jinhua People’s Hospital, Jinhua 321000, China; 2.Jinhua Institute of Food & Drug Inspection and Testing, Jinhua 321000, China; 3.Department of Clinical Medical Laboratory,Jinhua People’s Hospital, Jinhua 321000, China; 4.Department of Pathology, Jinhua People’s Hospital, Jinhua 321000, China
CHENG Zhenyu,SHENTU Huasong,CHEN Yihua, et al. The protective effect of UCF-101 on neurological function of rats with subarachnoid hemorrhage[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(11): 906-910,914.
Abstract：Objective: To examine the protective effect of UCF-101 on neurological function of rats with subarachnoid hemorrhage (SAH). Methods: A total of 90 rats were randomly assigned to Sham group, SAH group and UCF-101 group, with 30 rats in each group containing. The rat SAH animal model was established via double autologous blood injection into cisterna magna. Rats in the UCF-101 group were administrated intraperitoneally with UCF-101 (3.0 μmol/kg body weight). The western-blotting assay was used to determine the expressions of pro-caspase-3, pro-caspase-9, cleaved PARP, caspase-3 and caspase-9 in brain tissues of 10 rats in each group. The TUNEL staining was performed to investigate apoptotic neuronal cells in 10 rats of each group. Escape latency via the place navigation of Morris water maze and Kaoutzanis M’s neurobehavioral score were recorded of 10 rats from each group. Results: The brain tissue expressions of pro-caspase-3, pro-caspase-9, cleaved PARP, caspase-3 and caspase-9, percentage of apoptotic neuronal cells as well as escape latency of rats in SAH group were significantly higher than those of rats in Sham group (all P<0.05); while Kaoutzanis M’s neurobehavioral score were substantially lower in SAH group than in Sham group(P<0.05). As compare with SAH group, all the preceding variables were remarkably reversed to different extents in UCF-101 group (all P<0.05). Conclusion: UCF-101 can markedly inhibit the neuronal cellular apoptosis of SAH rats, and improve their cognitive function and neurobehavioral ability, and thereby exert a protective effect on neurological function of SAH rats.
