Primary research of the relationship between HCMV infection and gastric cancer
1.Department of Gastroenterological Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Department of Rheumatology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 3.Department of Gastroenterology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 4.Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, 325035
JIN Jinji1,TU Jianxin2,WU Ming3, et al. Primary research of the relationship between HCMV infection and gastric cancer[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(10): 703-707.
Abstract：Objective: To explore the correlation between HCMV infection and gastric cancer. Methods: The specific IgG and IgM antibodies of HCMV in serum of gastric cancer group (n=80) and health control group (n=80) were detected using the chemiluminesence immunoassay. HCMV infection in gastric cancer tissues and corresponding normal tissues were determined through the detection of UL55 gene by Nested PCR. The relationships between HCMV infection and clinical features of gastric cancer were further analyzed. Results: The positive rate of HCMV-IgG and IgM were 95% (76/80) and 6.25% (5/80) in gastric cancer group, respectively. Meanwhile, the positive rate were 97.5% (78/80) and 3.75% (3/80) in health control. There was no statistically significant difference between the gastric cancer and health control group in HCMV-IgG, IgM detection. However, there was a significant difference between gastric cancer tissues and corresponding normal tissues in HCMV detection. The positive rate of HCMV UL55 was 50% (11/22) in gastric cancer tissue samples, whereas 9.09% (2/22) in corresponding normal tissues through Nested PCR detection. In addition, There was no obvious association between HCMV infection and TMN stage, pathological differentiation type and the size of tumor. Conclusion: It is the first time to report the HCMV infection in gastric cancer tissues, and the HCMV may preferentially infect the neoplastic epithelium than corresponding normal epithelium, this research suggests the HCMV infection may participate in the occurrence and development stages of gastric cancer.
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