Protective effect of agaricus blazei polysaccharide on LPS-induced injury of human umbilical vein endothelial cells and its mechanism
CHEN Qin1, CHEN Lili1, ZHOU Aiming1, HUANG Xucai2, PAN Jingye1.
1.Department of Intensive Care, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China; 2.Department of Intensive Care, the Third People’s Hospital of Yueqing, Wenzhou 325604, China
CHEN Qin,CHEN Lili,ZHOU Aiming, et al. Protective effect of agaricus blazei polysaccharide on LPS-induced injury of human umbilical vein endothelial cells and its mechanism[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(11): 884-889,895.
Abstract：Objective: To investigate the protective effect of Agaricus blazei Murrill polysaccharide (ABP) on the injury of human umbilical vein endothelial cells (HUVECs) induced by lipopolysaccharide (LPS) and its possible mechanism. Methods: The cell damage model was constructed with LPS at a final concentration of 1 μg/mL and divided into control group, LPS group, and ABP intervention group (10, 20, 50 μg/mL). CCK-8 method was used to detect the effects of ABP on HUVECs. Cytotoxicity, ELISA, Western blot and RT-PCR were used to detect the expression of inflammatory factors and cell adhesion molecules in each group. Western blot and immunofluorescence were used to detect the expression of proteins related to the NF-κB signaling pathway and nuclear translocation in each group. Results: The relative expressions of TNF-α, IL-6, COX-2, iNOS, ICAM-1, VCAM-1, p-p65/p65, p-IκB/IκB protein in LPS group were significantly higher than those in control group (P<0.01). With the increase of drug concentration, the expression of above proteins in ABP group were significantly lower than that in LPS group (P<0.05). RT-PCR detection showed that TNF-α, IL-6, COX-2, iNOS, ICAM-1 and VCAM-1 mRNA expressions in LPS group were significantly higher than those in control group (P<0.01). With the increase of drug concentration, the expression of above-mentioned mRNA expressions in ABP group were significantly lower than that in LPS group (P<0.05). Conclusion: ABP can inhibit the expression of inflammatory factors and cell adhesion molecules induced by LPS to reduce the damage of HUVECs. The mechanism may be related to the inhibition of the activation of NF-κB signaling pathway in cells.
