Proteomic analysis by a comparison in protein difference between porcine and human decellularized nerve matrices using
JIANG Yongsheng1, ZHANG Mengpei2, HAN Chunchan1, HUANG Lijiang2
1.Science and Education Management Center, the Affiliated Xiangshan Hospital of Wenzhou Medical University, Ningbo 315700, China; 2.Department of Gastroenterololgy, the Affiliated Xiangshan Hospital of Wenzhou Medical University, Ningbo 315700, China
JIANG Yongsheng,ZHANG Mengpei,HAN Chunchan, et al. Proteomic analysis by a comparison in protein difference between porcine and human decellularized nerve matrices using[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(11): 867-872.
Abstract：Objective: To analyze the proteomic differences between porcine and human peripheral nerves after acellularization, which will provide the porcine decellularized material as a promising platformfor repairing peripheral nerve injury (PNI). Methods: All proteins in the porcine decellularized nerve matrix (pDNM) and human decellularized nerve matrix (hDNM) were extracted by grinding and enzymolysis. Then, the differentially expressed proteins (DEPs) were done with GO and KEGG pathway analysis using TMT labeled nano-HPLC-MS/MS technology. Results: A total of 1 448 corresponding proteins were identified, of which 456 and 170 proteins in pDNM manifested respective upregulation and downregulation, when compared with hDNM. Moreover,pDNM also contained more extracellular matrix (ECM) related proteins. GO functional annotation results suggested that the upregulated expressing proteins involved GO terms for cellular component were commonly enrichedin growth cone, axon and neuronal cell body; involved GO terms for biological process were generally associated with cell migration, cytoskeleton organization and axon development; andinvolved GO terms for molecular function were mainly related with molecular function regulator, structural constituent of cytoskeleton and motor activity. KEGG analysis also revealed that most of these DEPs participated in the regulation of actin cytoskeleton, axon guidance and ECM-receptor interaction. Conclusion: pDNM, thanks to its abundant structural and functional proteins, is a potential optional substitute for repairing PNI.
