HE Linjie,LIAO Xin,PAN Yinan, et al. Preparation of acellular cartilage microfilaments/thermosensitive hydrogel composite scaffold with sustained release of TGF-β3[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(10): 815-819.
Abstract：Objective: To explore the methods for preparation of acellular cartilage microfilaments/thermosensitive hydrogel composite scaffold which is injectable and can release TGF-β3 sustainedly. Methods:Decellularized cartilage microfilaments with sustained release of TGF-β3 were prepared by the method of detergent-enzyme chemical digestion and layer-by-layer self-assembly technology. After being mixed with thermosensitive hydrogel, the acellular cartilage microfilaments/thermosensitive hydrogel composite scaffold was prepared. The sustained release of TGF-β3 was detected by ELISA kit. Then the bone marrow mesenchymal stem cells (BMSCs) were co-cultured with the composite scaffold and observed under microscope. The expression of Col II, AGG and Sox9 were detected by the method of Western blot. Results: The composite scaffold was in sol state at low temperature, and changed to gel state when the temperature increased to 37 ℃. The TGF-β3 in the composite scaffold could be released slowly and effectively for about 25 d. BMSCs grew well on the composite scaffold, and the cells were similar to chondrocytes. And the expression of Col II, AGG and SOX9 increased significantly (P<0.05). Conclusion: The acellular cartilage microfilaments/thermosensitive hydrogel composite scaffold, injectable and able to release TGF-β3 sustainedly, was successfully prepared, which can induce chondrogenic differentiation of BMSCs.
