Metabolic alterations in the contralateral cerebellum of rats with transient and permanent middle cerebral artery occlusion
XIA Nengzhi1, HU Zilong1, HUANG Qun1, ZHENG Hong2, GAO Hongchang2, YANG Yunjun1,LI Jiance1.
1.Department of Radiology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China; 2.Institute of Metabonomics & Medical NMR, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China
XIA Nengzhi,HU Zilong,HUANG Qun, et al. Metabolic alterations in the contralateral cerebellum of rats with transient and permanent middle cerebral artery occlusion[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(10): 789-793,799.
Abstract：Objective: To investigate metabolic alterations in contralateral cerebellum of the transient(tMCAO) and permanent middle cerebral artery occlusion (pMCAO) rats by 1H NMR-based metabolomics.Methods: The rats were divided into CON group (n=12), pMCAO group (n=29) and tMCAO group (n=23). Brain MRI scan was performed and the contralateral cerebellum was detected by 1H NMR with pattern recognition and quantitative analysis of metabolites. Results: Although MRI showed no obvious abnormalities, the metabolic patterns of contralateral cerebellum were significantly changed in three groups. Compared with CON group,alanine (Ala) and lactate (Lac) decreased significantly, while γ-aminobutyric acid (GABA), glutamine (Gln),myo-inositol (m-Ins), aspartate (Asp) and creatine (Cre) increased significantly for contralateral cerebellum in pMCAO group (P<0.05); Ala and Lac decreased significantly, while glutamate (Glu), m-Ins, Asp and Cre increased significantly for contralateral cerebellum in tMCAO group (P<0.05). Gln of contralateral cerebellum decreased significantly in tMCAO group compared with pMCAO group (P<0.05). Conclusion: The metabolism of the contralateral cerebellum was significantly altered in the transient and permanent cerebral ischemia, mainly involving energy metabolism, neurotransmitter metabolism and glial cell activity. Different ischemic time showed variant impact on Gln-Glu-GABA cycle of cerebellum.
