Expression of a-1, 2 fucosyltransferase II in lung squamous cell carcinoma and its effect on proliferation of lung squamous cell carcinoma cells
WANG Weijie1, FAN Sairong2, ZHANG Guanghui1, SUN Haiting1, SHI Guofu1, MA Xuan1, ZHU Chengchu3
1.Department of Thoracic Surgery, the Affiliated Xiangshan Hospital of Wenzhou Medical University, Ningbo 315700, China; 2.School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, China; 3.Department of Thoracic Surgery, the Affiliated Taizhou Hospital of Wenzhou Medical University, Taizhou 317000, China
WANG Weijie,FAN Sairong,ZHANG Guanghui, et al. Expression of a-1, 2 fucosyltransferase II in lung squamous cell carcinoma and its effect on proliferation of lung squamous cell carcinoma cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(8): 662-665.
Abstract：Objective: To investigate the expression of a-1, 2 fucosyltransferase II (FUT2) in human lung squamous cell carcinoma (LUSC) and FUT2 effect on the cell proliferation of LUSC. Methods: UALCAN database was used to analyze the expression and clinical significance of FUT2 in lung squamous carcinoma; the shRNA plasmid of FUT2 was transferred into LUSC’s cell line (H226) by transient transfer technique to construct the knockdown expression of FUT2; CCK-8, colony formation and Western blot were used to investigate the effect of FUT2 on cell proliferation of LUSC-cell growth, monoclonal cell mass formation and the expression of proliferator PCNA protein. Results: The expression of FUT2 in LUSC’s tumor tissue was higher than that in normal control tissue, especially in the early and middle stages (P<0.05). But the expression of FUT2 had no relations with LUSC patients’ age and sex. In addition, the lower expression of FUT2 inhibited the growth and formation of monoclonal cell clusters as well as repressed the expression of PCNA protein in LUSC’s cell line, which all has statistical significance (P<0.05). Conclusion: FUT2 is highly expressed in LUSC and could promote the cell proliferation of LUSC, which might become a diagnostic index of LUSC.
